环维黄杨星D对高血压大鼠脑缺血GAP-43与Neurocan表达的影响  

Effects of the CVB-D on expression of the cerebral ischemia GAP-43 and Neurocan in the hypertensive rat

作　　者：谭峰[1] 顾卫[1] 万赛英[1] 吴海科[1] 王金良[1] 张炘[1] 黄任锋[1] 孙景波[2] 成家茂[3] 

机构地区：[1]广州中医药大学附属医院佛山市中医院神经内科,广东佛山528000 [2]广东省中医院中心实验室,广东广州516000 [3]中山大学神经生物学实验室,广东广州510080

出　　处：《中风与神经疾病杂志》2009年第1期86-90,共5页Journal of Apoplexy and Nervous Diseases

基　　金：省中医药管理局资助项目(NO.103142)

摘　　要：目的观察中药单体环维黄杨星D(CVB-D)对易卒中型肾血管性高血压大鼠(RHRSP)脑缺血-复流不同时间脑组织生长相关蛋白-43(GAP-43)与神经粘蛋白(Neurocan)表达的影响。方法采用环形银夹使SD大鼠双侧肾动脉狭窄,制成RHRSP,再用线栓法制成一侧大脑中动脉闭塞(MCAO)模型。用免疫组化方法观察CVB-D对MCAO大鼠脑缺血2h后复流1、7、14、30d脑组织GAP-43与Neurocan表达的影响,并与生理盐水组对照。结果缺血2h后再灌注1d,对照组缺血周围半暗区出现GAP-43阳性细胞,7d明显增多,14d减少,30d明显减少,各时间点阳性细胞数表达差异有显著性意义(P<0.01);治疗组GAP-43阳性细胞数表达在各时间点较对照组显著增加(P<0.01)。Neurocan阳性细胞数表达对照组在缺血再灌注1d出现,7d明显增多,14d达高峰,30d时下降,但仍高于假手术组水平(P<0.05);治疗组neurocan阳性细胞数表达在缺血再灌注7、14、30d则较对照组显著减少(P<0.01)。结论CVB-D上调RHRSP脑缺血区GAP-43阳性细胞数表达与下调Neurocan表达的作用,可能是其促进脑损伤区中枢神经修复的重要机制之一。Objective To observe the effects of the CVB-D on the expression of GAP-43 and Neurocan during the different time of the cerebral ischemia and reperfusion in Stroke-prone-Renovascular-Hypertensive-Rats. Methods Stroke-prone-Renovascular-Hypertensive-Rats（RHRSP） models were made by bilaterally narrowing the renal artery with silver loop clips and the models of rat middle cerebral artery occlusion（ MCAO）were created by stringing middle cerebral artery occlusion in RHRSP. The effects of the CVB-D on the expression of GAP-43 and Neuroean in the rat cerebral tissue were detected by immunohistochemistry after 2-hour ischemic reperfu.sion and 1-day,7-day 14-day and 30-day reperfusion, which would be compared with the control group. Results The negative GAP-43 cells were found in the semidarkness area after 2-hour isehemic and 1-day reperfusion, which was decreased after 14-day, and reduced siguificanfly after 30-day. There was a significant difference between the two groups（ P 〈 0.01 ）. Contrast to the control group, the expression of negative GAP-43 in CVB-D group were obviously increased at different time. The expression of negative Neurocan cells in control group were found after 1 -hour isehemia and reperfusion. It was increased significantly after 7-day and reached the maximum after14-day reperfusion. Contrarily, it was decreased after 30-day but still higher than that in sham-operated group（ P 〈 0.05 ）. Contrast to the control group, the number of negative neurocan cell in CVB-D group was decreased after and 1- day and 14-day isehemic and repeffusion,as well as 30-day ischemic and reperfusion（P 〈0.01 ）. Conclusion The CVB- D made an important role in the process of the ischemic cerebral damage from the aspect of the upper regulation to the expression of negative number of GAP-43 cell and down regulation to the expression of negative number of neurocan cell, which is one of the vital mechanism for the recovery of central nerve in the cerebral ischemic area.

关 键 词：脑缺血-复流 GAP-43 NEUROCAN 环维黄杨星D 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]