莱菔硫烷对血管内皮细胞粘附分子表达影响  

Effects of sulforaphane on expressions of ICAM-1 and VCAM-1 mRNA induced by LPS and nuclear translocation of NF-κB in vascular endothelial cell

作　　者：林娜[1] 单毓娟[1] 赵瑞芳[1] 袁超[1] 王舒然[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,哈尔滨150081

出　　处：《中国公共卫生》2009年第3期329-331,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30571558);黑龙江省自然科学基金(D2005-44)

摘　　要：目的研究莱菔硫烷对脂多糖诱导的人脐静脉内皮细胞(ECV304)的细胞中粘附分子(ICAM-1)和血管细胞粘附分子-1(VCAM-1)mRNA表达影响以及转录机制。方法采用ECV304细胞为研究对象,用实时定量PCR方法(real-tim e PCR)检测ICAM-1和VCAM-1的mRNA表达水平,用免疫荧光法检测转录因子(NF-κB)向核内转位的情况。结果用5,10,20μmol/L莱菔硫烷作用再用脂多糖刺激,细胞中ICAM-1mRNA表达量下降,分别为对照组的2.3,1.5和1.1倍,呈一定剂量-效应关系,与脂多糖组比较,差异均有统计学意义(均P<0.05);5μmol/L莱菔硫烷能使脂多糖诱导的VCAM-1 mRNA表达降低为1.4倍,而10,20μmol/L莱菔硫烷则完全抵消了脂多糖对VCAM-1 mRNA的诱导,2组VCAM-1 mRNA的表达均降低为对照组的0.4倍,与脂多糖组比较,差异均有统计学意义(均P<0.05);10μmol/L莱菔硫烷作用后,细胞核中绿色荧光明显弱于脂多糖单独处理组,表明莱菔硫烷可抑制脂多糖诱导的NF-κB的核转位。结论莱菔硫烷可通过阻断NF-κB的核转位来抑制脂多糖诱导的ECV304细胞ICAM-1和VCAM-1 mRNA的表达。Objective To study the effects of sulforaphane on the expressions of ICAM-1 and VCAM-1 mRNA induced by lipopolysaccharide （LPS） in vascular endothelial cell ECV304 and its transcriptional mechanism. Methods The levels of mRNA of ICAM-1 and VCAM-1 were determined by quantitative real - time PCR and nuclear translocation of NF- κB was measured by immunofluorescence hybridization. Results Treated with 5,10,20 μmol/L sulforaphane followed by LPS , the expressions of ICAM-1 mRNA in ECV304 were deminished to 2. 3,1.5 and 1.1 fold of the level of the control, respectively. Compared with LPS, sulforaphane significantly inhibited the expression of ICAM-1 mRNA in dose-dependent manner（ P 〈 0.05）. 5 μmol/L sulforapliane down-regulated expression of VCAM-I mRNA induced by LPS to 1.4 fold , while 10,20 μmol/L sulforaphane completely eliminated the stimulation and the level of VCAM-1 mRNA was only 0.4 fold of the control（ P 〈 0. 05 ）. The weakness of the strength of green fluorescence in nuclear indicated that 101μmol/L sulfora- phane could block the translocation of NF - κB. Conclusion Sulforaphane down - regulates the expression of ICAM-1 and VCAM-1 mRNA through blocking the translocation of NF-κB into nucleus.

关 键 词：莱菔硫烷 细胞中粘附分子(ICAM-1) 血管细胞粘附分子-1(VCAM-1) 转录因子(NF-κB) 

分 类 号：R151.41[医药卫生—营养与食品卫生学]