低氧对人胚肺成纤维细胞增殖及I型前胶原基因表达的影响  

Effect of Hypoxia on Proliferation and α(I) Procollagen Gene Expression by Human Fetal Lung Fibroblasts

作　　者：赵磊[1] 杨红[1] 郭晓明[1] 陈祥银[1] 严仪昭[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所,北京100005

出　　处：《中国医学科学院学报》1998年第2期108-113,共6页Acta Academiae Medicinae Sinicae

基　　金：国家"八五"科技攻关项目(85-915-03-05);中国医学科学院中国协和医科大学青年科学基金(9532005)

摘　　要：目的阐明低氧在血管壁构形重组中的作用及川芎嗪的疗效机理。方法用细咆计数法及非放射性细胞增殖检测法观察2%低氧对人胚肺成纤维细胞增殖的影响;用Northern印迹杂交法检测2%低氧对人胚肺成纤维细胞Ⅰ型前胶原基因表达的作用及川芎嗪对基因表达的影响。结果低氧24.48及72h能明显促进人胚肺成纤维细胞增殖;低氧24h Ⅰ型前胶原基因表达明显升高;川芎嗪(30μg/ml及10μg/ml)可抑制上述基因表达的升高。结论低氧对人胚肺成纤维细胞有直接利激作用,通过促进其增殖及前胶原基因表达而影响肺血管构形重组和损伤组织的修复。The effect of 2% hypoxia on the proliferation and α_1 (Ⅰ) procollagen gene expression by human fetal lung fibroblast was studied. Methods Cell proliferation was determined by cell counting and nonradioactive cell proliferation assay method, α_1 (Ⅰ) procollagen gene expression was detected by Northern blot hybridization analysis. Results Cell number and 490 nm OD expressing low oxygen values were markedly increased by the 24, 48 and 72h exposure to 2% hypoxia respectively. An obvious increase of the α_1 (Ⅰ) procollagen mRNA expression was detected after 24 h exposure to 2% hypoxia. The increase of gene expression could be inhibited by Ligustrazin (30 μg/ml, 10 μg/ml). Conclusions 2% hypoxia plays an important role in the outcome of wound healing and pulmonary vascular remodeling by affecting the function of human fetal lung fibroblasts.

关 键 词：低氧 成纤维细胞 增殖 Ⅰ型前胶原基因 

分 类 号：R364.4[医药卫生—病理学]