HPLC法测定降脂减肥片中葡萄糖苷含量  

Determination Of 2,3,5,4'-Tetrahydroxystilbene-2-O-B-D-Glucoside In Jiangzhijiafei Tablet By HPLC

作　　者：郝来义[1] 牛俊瑞[1] 李春梅[1] 

机构地区：[1]内蒙古医学院第三附属医院药剂科,内蒙古包头014010

出　　处：《疾病监测与控制》2009年第3期147-147,146,共2页Journal of Diseases Monitor and Control

摘　　要：目的建立用高效液相色谱法(HPLC法)测定降脂减肥片中2,3,5,4'-四羟基二苯乙烯-2-0-β-D葡萄糖苷含量的方法。方法采用HPLC法在波长320nm处进行含量测定,同时进行方法学验证。结果2,3,5,4'-四羟基二苯乙烯-2-0-β-D-葡萄糖苷在进样量为0.3672～3.672u g范围内峰面积与含量呈线性关系(R=0.9993,n-6)。平均回收率为100.3%。RSD为1.3%。结论本法简便、快速、重现性好,可作为于降脂减肥片中的2,3,5,4'-四羟基二苯乙烯-2-0-β-D葡萄糖苷的定量分析方法。Objective To establish the method of determining 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside in Jiangzhijianfei Tablet by HPLC. Method HPLC was used ,the representative component,at wavelength of 320nm.The method was validated. Results 2,3,5,4＇ -Tetrahydroxystilbene-2-O-β-D-glucoside showed a good linear relationgship at a range of 0.3672-3.672μg（r=0.9993,n=6）.The average recovey was 100.3 %, RSD=1.3%,n=5. Conclusion The method was simple,accurate,realizable and reproducible.It can be use effectively for determination 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside in Jiangzhijiafei Tablet.

关 键 词：HPLC法 降脂减肥片 2 3 5 4’ 四羟基二苯乙烯2-O-β-D葡萄糖苷 含量测定 

分 类 号：R914.1[医药卫生—药物化学]