Hedgehog信号转导途径对乳腺癌MDA-MB-231细胞增殖与凋亡以及对CycinD1表达的影响  

Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231

作　　者：黄伟[1] 张徽[1] 姜小良[1] 刘香丽[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016

出　　处：《重庆医科大学学报》2009年第1期45-48,共4页Journal of Chongqing Medical University

摘　　要：目的:本实验通过Hedgehog(HH)信号转导途径的抑制剂Cyclopamine作用于人乳腺癌MDA-MB-231细胞,研究Hedgehog信号转导途径对乳腺癌MDA-MB-231细胞增殖与凋亡以及对CycinD1表达的影响。方法:以MDA-MB-231细胞为研究对象,应用Cyclopamine处理后,用MTT方法检测细胞增殖活性的改变;用流式细胞术检测细胞周期与凋亡的情况;RT-PCR检测GLI1、CyclinD1的mRNA的变化;用免疫细胞化学术检测细胞中的CyclinD1蛋白表达的变化。结果:与对照组相比,Cyclopamine可显著抑制MDA-MB-231细胞的生长并呈时效-量效关系;流式细胞术检测MDA-MB-231细胞,发现Cyclopamine能提高细胞周期G0/G1期的比例,48h后出现明显的"亚G1"峰;GLI1,CyclinD1的mRNA以及CyclinD1蛋白的表达降低。结论:乳腺癌MDA-MB-231细胞有Hedgehog信号转导途径的激活;Hedgehog信号转导途径的抑制剂Cyclopamine能明显抑制MDA-MB-231细胞生长并能诱导其凋亡,减少CyclinD1的表达。Objective： To research the effects of HH singaling pathway on proliferation,apoptosis and CyclinD1 expression of breast cancer MDA-MB-231 cells by MDA-MB-231 cells treated with Cyclopamine,a HH singaling pathway inhibitor.Methods： MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;Cell cycle distribution and apoptosis were analysed by flow cytometry（FCM ）.The mRNA levels of GLI 1, CyclinD1 were measured by reverse transcfiption-polymerase chain reaction （RT-PCR） ;The protein level of CyelinD1 was detected by immunostaining of cell lines. Results： Compared with control group, Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose-and time-dependent manner;By FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48 h the sub- G1 peak appeared. The mRNA levels of GLI1 ,CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusion： The HH signaling pathway was activeted in MDA-MB-231 cells. HH signaling pathway inhibitor, Cyclopamine, could inhibit the proliferation, induce the apoptosis and reduce the expression of CyclinD 1 in MDA-MB-231 cells.

关 键 词：HEDGEHOG GLI1 CYCLIND1 信号转导 CYCLOPAMINE 

分 类 号：R361.3[医药卫生—病理学]