腹水来源卵巢癌细胞系的建立及其生物学特性  

Establishment and biological characteristics of a human ovarian carcinoma cell line originated from peritoneal effusion

作　　者：程继文[1] 蔡捷[2] 邝晓聪[3] 陈心秋[4] 唐步坚[4] 黄清华[4] 刘燕[4] 李力[4] 

机构地区：[1]广西医科大学附属肿瘤医院泌尿外科,广西南宁530021 [2]广西医科大学医学实验中心,广西南宁530021 [3]广西医科大学病理生理学教研室,广西南宁530021 [4]广西医科大学附属肿瘤医院妇瘤科,广西南宁530021

出　　处：《癌症》2009年第3期232-235,共4页Chinese Journal of Cancer

摘　　要：背景与目的:体外建立卵巢癌细胞系有利于深入了解卵巢癌干细胞的生物学特性及抗癌药物的筛选。本研究中我们以人卵巢癌患者腹水为材料建立卵巢癌细胞系,并进一步探讨其基本生物学特性。方法:体外分离卵巢癌患者腹水中的肿瘤细胞,纯化并传代培养,观察细胞形态学变化,绘制细胞生长曲线,计算细胞倍增时间,进行染色体核型分析。收集体外培养上清,进行放射免疫法检测相关激素状况和肿瘤标志物表达。动物实验观察细胞成瘤性以及瘤体的病理形态。结果:该卵巢癌细胞系已在体外培养与传代传110余代,历时近2年,透射电镜下可观察到卵巢癌细胞有显著的异形核,体外生长的倍增时间为40.8h,染色体核型分析为超二倍体,染色体数目分布为63～120条。第80代细胞培养上清中雌二醇为45.0pg/mL、睾酮0.03ng/mL、孕酮未测出,CA125为4.49U/mL,CA19-9为4.09U/mL。第51代细胞接种于裸鼠后有皮下成瘤性,为低分化腺癌。结论:腹水来源的卵巢癌细胞在体外培养已是永生化细胞系,并具有明显的恶性细胞的生物学特征。Background and Objective： Establishing ovarian cancer cell lines will benefit researches on biological characteristics of ovarian cancer stem cells and antitumor drugs. This study was to establish a human ovarian carcinoma cell line from peritoneal effusion, and explore its biological characteristics. Methods： Cells were isolated from peritoneal effusion of an ovarian carcinoma patient, purified and cultured in vitro. The morphology of the cells was observed under electromicroscope. The growth curve of cells was drawn to calculate cell doubling time （TD）. Cell karyotype was analyzed. The levels of sex hormone, and the expression of CA125 and CA19-9 in cell culture supernate were detected by radioimmunoassay. The cells were transplanted into nude mice to observe tumor formation. Results： A new ovarian carcinoma cell line was established, which had been maintained in vitro for over 100 passages in two years. Abnormal nuclei were observed under electromicroscope. TD was 40.8 h. The karyotype of the cells was hyperdiploid with 63-120 chromosomes. The level of estradiol in cell culture supernate was 45.0 pg/mL; the level of testosterone was 0.03 ng/mL; pregnendione was undetected; the level of CA125 was 4.49 U/mL; the level of CA19-9 was 4.09 U/mE Poorly differentiated ovarian adenocarcinoma was formed subcutaneously in nude mice after transplantation. Conclusion： The new ovarian carcinoma cell line is proved to be an immortalized and malignant cell line.

关 键 词：卵巢肿瘤 细胞培养 腹水癌细胞 细胞系建立 生物学特性 

分 类 号：R737.31[医药卫生—肿瘤]