非小细胞肺癌患者围手术期循环血中癌细胞的Pin1表达及意义  

Expression and significance of Pin1 mRNA in blood samples from non-small cell lung cancer patients during perioperative period

作　　者：艾正华[1] 张位星[1] 罗万俊[1] 黄凌瑾[1] 潘艺 

机构地区：[1]中南大学湘雅医院心胸外科,湖南长沙410008 [2]中信湘雅生殖与遗传专科医院分子遗传学实验室,湖南长沙410008

出　　处：《癌症》2009年第3期268-273,共6页Chinese Journal of Cancer

摘　　要：背景与目的:Pin1(peptidylprolyl cis/trans isomerase NIMA-interacting1)的催化反应是一条改变磷酸化蛋白功能的信号通道,在肿瘤的发生中起着重要作用,被称为肿瘤发生的催化分子。本研究目的为分析Pin1在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者围手术期循环血中的表达及意义。方法:接受根治性手术的原发性NSCLC患者26例,术前按预定的结扎血管顺序分为先结扎肺静脉组和先结扎肺动脉组,分别采集其术前(麻醉后)外周血标本、结扎肺静脉后近心端和远心端血标本及术后第七日外周血标本。选择10例需手术治疗的肺部良性疾病患者作为对照,以10例健康人作为阴性对照。采用实时荧光定量逆转录聚合酶链反应法定量检测患者循环血中的Pin1 mRNA表达,并分析其与NSCLC临床病理特征的关系。结果:NSCLC患者循环血中Pin1 mRNA表达量显著高于肺部良性病变患者和健康人,NSCLC患者循环血中的Pin1 mRNA表达量是健康人中的1.69～34.78倍。Pin1 mRNA表达量与和癌症分期、淋巴结转移有关(P=0.043,P=0.038)。肺静脉远心端的表达量高于近心端的表达量(P=0.019),而先结扎肺静脉组和先结扎肺动脉组无显著性差异(P=0.082,P=0.106)。手术后一周的Pin1 mRNA表达显著低于术前和术中(P=0.031)。结论:Pin1在NSCLC患者血液循环中高表达,可能作为肺癌诊断治疗的肿瘤标志物。Background and Objective. The catalytic reaction of Pin1 （peptidylprolyl cis/trans isomerase NIMA-interacting 1） is a signal pathway for changing the functions of phosphorylated proteins, which plays an important role in tumorigenesis. Pin1 is called the catalytic molecule for tumorigenesis. This study was to detect the expression of Pin1 mRNA in blood samples from non-small cell lung cancer （NSCLC） patients, and explore its significance. Methods： Twenty-six NSCLC patients who underwent radical resection were assigned to pulmonary artery first ligation group （PA- first group） and pulmonary vein first ligation group （PV-first group）. The blood samples were collected before operation （after anesthesia）, during operation from the proximal part and distal part of the pulmonary vein when it was ligated, and at 7 days later. Additionally, ten patients with benign lung disease who underwent resection served as disease control, and ten healthy subjects served as negative control. The expression of Pin1 in the blood samples was detected by real-time reverse transcription-polymerase chain reaction （RT-PCR）. Results. The mRNA level of Pin1 was obviously higher in blood samples from NSCLC patients than in those from benign lung disease patients and healthy subjects, it in NSCLC group was 1.69-34.78 times of that in negative control group. It was associated with lymph node metastasis and clinical stage of NSCLC （P=0.043, P=0.038）. The mRNA level of Pin1 was significantly higher in the distal part of the pulmonary vein than in the proximal part （P=0.019）, and was significantly lower at 7 days after operation than before operation （P=0.031）. There was no significant difference between PA-first group and PV-first group （P=0.082, P=0.106）. Conclusion： Pin1 is overexpressed in circulation of NSCLC, and may be used as a tumor marker or as a target for cancer therapy.

关 键 词：PIN1 肺肿瘤 real—time PCR 肿瘤标志物 

分 类 号：R734.2[医药卫生—肿瘤]