原发性感染、再感染根管中古细菌的RT-PCR分析  

Detection of archaea in root canal of primary and secondary endodontic infections by RT-PCR

作　　者：姜云涛[1,2] 夏文薇[1,2] 梁景平[1,2] 闫培芳[1,2] 马善奋 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院牙体牙髓科 [2]上海市口腔医学研究所.上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2009年第2期84-87,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30801291);上海市教委课题资助项目(jdy-07060)

摘　　要：目的:建立根尖周炎感染根管中古细菌分布的基线信息,初步探讨古细菌与根管感染及再感染发生的相关性。方法:收集原发性根尖周炎感染根管(31例)及根管治疗失败再感染根管样本(17例),提取总RNA并逆转录为cDNA,古细菌通用引物PCR扩增,比较分析两组样本中古细菌的检出率。结果:古细菌的检出率为27.08%,其中在原发性感染根管和根管治疗失败再感染根管中的检出率分别为35.48%和11.76%,两组之间没有统计学差异(P>0.05)。结论:古细菌稳定存在于不同类型感染根管中,提示古细菌可能参与根管感染的发生和发展。AIM ： To obtain the baseline information on the distribution of Archaea in the root canal of periapical periodontitis, and to explore the relationship between the distribution of primary and secondary endodontic infections. METHODS ： In this study, 48 samples of infected root canals were collected from 31 primary endodontie infected cases and 17 secondary endodontic infections respectively. After isolation of total RNA from the samples, eDNA was synthesized by reverse transcription, and determined by RT - PCR with Archaea universal PCR primers. RESULTS ： Detection rates of Archaea were 27.08%. To be specific, the detection rates of primary and secondary endodontic infections were 35.48% and 11.76% respectively. There was no significant difference between the two groups. CONCLUSION ： Arehaea can be detected in different groups of samples steady, which suggested that the Archaea may participate in the oceurrenee and development of endodontie infections.

关 键 词：感染根管 根管再感染 古细菌 逆转录聚合酶链反应 

分 类 号：R781.4[医药卫生—口腔医学]