酪蛋白空肠灌注对急性坏死性胰腺炎大鼠胰腺外分泌的影响  

Effect of jejunal casein perfusion on pancreatic exocrine secretion in experimental acute necrotic pancreatitis rats

作　　者：赵航[1] 李兆申[1] 黄丹丹[1] 龚燕芳[1] 高峻[1] 吴洪玉[1] 满晓华[1] 许爱芳[1] 

机构地区：[1]第二军医大学附属长海医院消化内科,上海200433

出　　处：《中华胰腺病杂志》2009年第1期12-14,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30370647）

摘　　要：目的观察空肠内灌注酪蛋白对急性坏死性胰腺炎（ANP）大鼠胰腺外分泌的影响，并初步探讨其神经机制。方法30只SD大鼠按随机数字法分为对照组、ANP组和肠内营养组。后2组以胰管内逆行注射牛磺胆酸钠法制作ANP模型。造模后24h空肠插管分别灌注酪蛋白溶液（肠内营养组）和生理盐水（对照组和ANP组），每15min收集胰液1次，共6次，记录胰液分泌量和检测胰液蛋白含量。取大鼠延髓孤束核，采用免疫组化法检测c—Fos蛋白表达。结果ANP组和肠内营养组组内各时间段之间胰液分泌量无显著差别，两组相应时间段之间胰液分泌量也无显著差别，但均显著低于对照组对应时间段的胰液分泌量（P〈0．05）。对照组、ANP组和肠内营养组空肠灌注过程中胰液蛋白含量水平稳定，不同时间段间无明显差异，但ANP组和肠内营养组空肠灌注后0～15min、15—30min、30～45min、75～90min时间段内胰液蛋白含量均低于对照组（P〈0．05）。肠内营养组灌注后延髓孤束核c-Fos蛋白表达阳性，而对照组和ANP组延髓孤束核c—Fos表达阴性。结论空肠内酪蛋白灌注可促进延髓孤束核c—Fos表达，但不增加胰液分泌量和胰液蛋白含量。Objective To investigate the effect of jejunal casein perfusion on pancreatic exocrine secretion in experimental acute necrotic pancreatitis （ANP） rats and analyze the neurumechanism that may be involved. Methods 30 SD rats were randomly divided into three groups （ control group, ANP group and ANP jejunal nutrition group）. Experimental ANP was induced by intra pancreatic duct injection of sodium taurocholate （STC）. Animals in ANP jejunal nutrition group were given jejunal casein perfusion 24h after model induction, while control group and ANP group received jejunal saline perfusion. Pancreatic juice was collected every 15 rain for six times and the volume of pancreatic juice and protein in pancreatic juice were detected. After jejunal nutrition c-Fos expression in nucleus tractus solitarii （NTS） was determined by immunohistochemistry method in three groups. Results There was no significant difference between the volume of pancreatic juice at different time points in ANP group and ANP jejunal nutrition group, however, these parameters were significantly lower than that of control group （ P 〈 0.05 ）. There was no significant difference among the 3 groups in the protein level in the pancreatic juice during jejunal nutrition infusion, however, during the periods of 0 - 15 min, 15 -30 min, 30 -45 min and 75 -90 min, the protein levels in the pancreatic juice in ANP and ANP jejunal nutrition group were lower than that of control group （P 〈 0.05）.After jejunal perfusion, c-Fos expression was found in ANP jejunal nutrition group but not found in ANP and control groups. Conclusions Jejunal casein perfusion enhanced NTS c-Fos expression, but did not increase the volume of pancreatic juice and protein.

关 键 词：胰腺炎 急性坏死性 肠道营养 孤束核 c—Fos 

分 类 号：R576[医药卫生—消化系统]