IL-6对人胰腺癌细胞生长及STAT3信号转导通路的影响  

Effect of interleukin.6 on growth and STAT3 signal transduction pathway in human pancreatic cancer cell line

作　　者：杨光[1] 张放[1] 江弢[1] 黄克俭[1] 曹俊[1] 黄陈[1] 裘正军[1] 

机构地区：[1]上海交通大学附属第一人民医院普外科,上海200080

出　　处：《中华胰腺病杂志》2009年第1期48-50,共3页Chinese Journal of Pancreatology

摘　　要：目的探讨IL-6对人胰腺癌细胞株Capan-2生长及STAT3信号转导途径的影响。方法采用MTr法检测不同浓度IL-6刺激后Capan-2细胞的增殖率；免疫细胞化学染色确定磷酸化STAT3（P．STAT3）在Capan-2细胞内的定位及IL-6刺激前后表达量的变化；流式细胞仪检测细胞的凋亡；Westernblot检测IL-6刺激前后Capan-2细胞中P—STAT3、bcl—xl、Cyclin D1蛋白表达量的变化。结果100ng／ml的IL-6作用Capan．2细胞后，细胞的增殖从1增加到4．965-t-0．18（P〈0．05）；细胞凋亡率从（3．21±0．23）％下降到（1．98±0．67）％（P〈0．05）；P-STAT3、bcl-xl、Cyclin D1蛋白表达明显升高（P〈0．05），且bcl-xl的表达与P—STAT3的表达呈正相关（r=0．985，P=0．015）；CyclinD1的表达与P-STAT3表达也呈正相关（r=0．914，P=0．036）。结论JAK／STAT信号转导途径的活化介导了IL-6对Capan-2细胞的增殖促进功能。Objective To investigate the effect of interleukin 6 （IL-6） on the growth and proliferation of human pancreatic cancer cell line Capan-2 and the signal transduction pathway. Methods MTT method was used to detect the effect of IL-6 of different concentrations on the growth and proliferation of Capan - 2 ceils ; cell apoptosis was detected by flow cytometry; the intracellular localization of phosphorylated STAT3 （ PSTAT3） was determined by immunocytocbemistry and western blot were used to detect P-STAT3, bcl-xl and Cyclin D1 in Capan-2 cells stimulated by IL-6. Results IL-6 （100 ng/ml） could remarkably promote the growth of Capan-2 cells from 1 to 4. 965 ± 0. 18 （ P 〈 0. 05 ） ; the percentage of apoptosis decreased significantly from （3.21 ± 0.23 ） % to （ 1.98 ±0.67 ） % （ P 〈 0.05 ） ; the expressions of P-STAT3, bel-xl, Cyclin D1 increased significantly （ P 〈 0.05 ） , and the expressions of bcl-xl was positively correlated with that of P-STAT3 （r = 0. 985, P = 0. 015 ） ; the expressions of Cyclin D1 was also positively correlated with that of P- STAT3 （r = 0.914, P = 0. 036）. Conclusions IL-6 activated JAK/STAT signal transduction pathway, which played an important role in the growth and proliferation of Capan-2 cells in the presence of IL-6.

关 键 词：胰腺肿瘤 白细胞介素6 信号转导及转录活化因子3 

分 类 号：R735.9[医药卫生—肿瘤] R392.11[医药卫生—临床医学]