正常男子和弱精子症者精子中3β-羟基甾体脱氢酶的研究  

Study on 3β-HSD of spermatozoa in fertile men and infertile patients with asthenospermia

作　　者：沈继云[1] 李福顺[2] 熊承良[1] 黄勋彬[1] 周洁玲[1] 陈文静[1] 夏文家[1] 

机构地区：[1]同济医科大学生殖医学中心,武汉430030 [2]武汉南威医学研究所

出　　处：《男科学报》1998年第1期14-15,共2页

摘　　要：目的：测定精子中3β-羟基甾体脱氢酶（HSD）。方法：采用酶组织化学方法对27例精子活率正常和20例精子活率低下的男子精子进行了研究。结果：3β-HSD主要分布在精子颈部，其着色面积不等，精子活率正常组3β-HSD着色面积为616．48±108．48μm2／100个精子，精子活率低下组为354．37±50．58μm2／100个精子，两组差异极显著（P＜0．001）。结论：3β-HSD可能通过某种途径间接参与精子的运动。Objectives and Methods: 3β-HSD of spermatozoa in 27 men with normal sperm motility and 20 men with asthenospermia was studied by method of enzymatic histochemstry. Results: 3β-HSD was mainly located in the neck of spermatozoa. There was a significant difference concerning the stainig area of sperm between two groups. The staining area in the group of normal sperm motility was 616. 48±108. 48 μm2/100 spermatozoa; that of the group of low sperm motility was 354. 37± 50. 58 μn2/100 spermatozoa. Significant difference was detected between the group of normal sperm motility and the group of low sperm motility (P＜ 0. 001). Conclusions: It suggested that 3β-HSD may be indirectly associated with sperm movement via an unknown pathway.

关 键 词：精子 酶学 3Β-HSD 弱精子症 

分 类 号：R698.202[医药卫生—泌尿科学] R446.19[医药卫生—外科学]