光化学损伤下大鼠视网膜超氧化物歧化酶基因mRNA表达的实验研究  

The experimental studies of expression of genes in light damaged rat retinas

作　　者：刘瑜玲[1,1,2] 严密[1,1,2] 刘柏林[1,1,2] 

机构地区：[1]华西医科大学第一临床医院眼科 [2]华西医科大学基础医学院分子生物学研究室

出　　处：《眼科》1998年第1期57-59,共3页Ophthalmology in China

摘　　要：通过建立大鼠视网膜光化学损伤模型，应用原位杂交技术对正常及光化学损伤大鼠视网膜超氧化物歧化酶基因ｍＲＮＡ表达情况进行动态观察。结果表明，视网膜组织各层均可见超氧化物歧化酶基因ｍＲＮＡ的表达；连续光照可使其表达水平迅速下降。原位杂交技术为研究视网膜基因表达状况的一种良好方法。The changes of expression of SOD mRNA in light damaged rat and normal rat retinas were studied in the rat model of retinal photic injury with the technique of in situ hybridization It was demonstrated that the hybridization signals of SOD mRNA were distributed in any layer of the retinas and the expression level of SOD mRNA dramatically decreased with the increase of light exposure time It is a good way to analyze the expression level of the gene of retinas with the technique of in situ hybridization

关 键 词：超氧化物歧化酶 基因 眼损伤 分子生物学 

分 类 号：R779.12[医药卫生—眼科]