鸭肠炎病毒糖蛋白gC基因克隆与序列分析  

Cloning,sequence analysis of glycoprotein C gene of duck enteritis virus

作　　者：刘峰源[1] 马波[1] 张杨[1] 赵姸[1] 王君伟[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2009年第2期5-7,共3页Chinese Journal of Veterinary Medicine

摘　　要：为了研究鸭肠炎病毒(duck enteritis virus,DEV)gC基因的特征,运用Tail-PCR的方法,克隆得到DEV的gC基因的全部和UL43基因的部分序列,共计2 718 bp。其中UL43基因的部分序列为600 bp;UL44基因全长1 296 bp,编码431个氨基酸。与12株已报道的具有全gC(UL44)同源基因序列的疱疹病毒参考株进行比较,有3个保守区域。系统发育进化分析发现其与α-疱疹病毒亚科中禽的疱疹病毒亲缘关系较近。To identify the features of DEV gC, the thermal asymmetric interlaced PCR was used to amplify the glycoprotein C （UL44） open reading frame （ORF） of the duck enteritis virus （DEV）, and a 2 718 bp DNA fragment was obtained, which covers the entire DEV gC（UL44） gene and partial UL43 gene. The partial UL43 gene was 600 bp. The UL44 gene was 1 296 bp and encoded a 431 amino acid peptide. Comparison of gC homologies among DEV and 12 reference herpesvirus strains showed that the gC of DEV had three conserved regions with alphaherpesviruses. Phylogenetic analysis of the gC gene of DEV showed that DEV was more closely related to avian herpesviruses than to other herpesviruses.

关 键 词：TAIL-PCR DEV GC 

分 类 号：S858.32[农业科学—临床兽医学]