鼻咽癌中EB病毒LMP1基因的序列变异发生机制探讨  

Study on sequence variations mechanism of epstein-barr virus LMP1 gene in nasopharyngeal carcinoma

作　　者：李珀[1] 何常[1] 徐恒天[1] 钟愉[1] 

机构地区：[1]贵阳医学院病理学教研室,550004

出　　处：《贵州医药》2009年第1期3-5,共3页Guizhou Medical Journal

基　　金：贵州省科技厅基金资助[2004NGY044]

摘　　要：目的检测贵州省鼻咽癌中EB病毒LMP1基因N-和C-末端区序列变异的热点,并探讨其产生的机制。方法收集贵州省贵阳医学院有明确病理诊断的鼻咽癌患者鼻咽新鲜活检标本30例。采用巢式聚合酶链反应(PCR)扩增EB病毒LMP1基因N-和C-末端区,用Xho I对N-末端区扩增产物进行酶切分析,从中选取2例患者N-末端区的扩增产物进行序列分析。同时检测C-末端区扩增产物30 bp缺失的情况。结果30例鼻咽癌组织EB病毒LMP1基因C-末端区存在两种基因型:缺失型(即30 bp缺失)和野生型,其中病例组有40%的突变率,对照组有10%的突变率,差异有显著性(P<0.05)。在本次研究中的30例鼻咽癌及随机收集贵阳医学院体格检查健康成人30例的外周血(EDTA抗凝)2 mL作对照组中EB病毒LMP1基因N-末端区都发生了突变,都存在原有酶切位点的缺失。结论鼻咽癌中EB病毒LMP1基因突变在鼻咽癌的发生发展中起重要的作用。Objective To detect the sequence variations frequently found within the N-and C-terminal regions of Epstein-Barr virus （EBV） LMP1 gene in nasopbaryngeal carcinoma （NPC） and to search for the underlying mechanisms. Methods Fresh tumor biopsy tissues were collected from 30 patients with NPC. The N-terminal region of EBV LMP1 gene was amplified with nested polymerase chain reaction （PCR）, followed by Xho I enzyme digestion, and taken two typical specimens of PCR products to analyze the DNA sequence. Amplified products of 30 base pairs in C-terminal regions were detected. Results There were two types of sequence variations frequently found within the C-terminal regions of Epstein-Barr virus （EBV） LMP1 gene in nasopharyngeal carcinoma （N-PC）, which were the del-LMP1 and wt-LMP1. The mutation rate of was 40% in 30 NPC cases, and the mutation rate was 10% in control group. There were statistics significance between them （P〈0. 05）. Sequence analysis showed that the both of NPC and control group had underwent mutation within the C-terminal regions of Epstein-Barr virus （EBV） LMP1 gene. Conclusion The gene mutation of LMP1 plays the important roles in the development of NPC.

关 键 词：鼻咽肿瘤 人 膜蛋白类 

分 类 号：R739.62[医药卫生—肿瘤]