氨氯地平对ox-LDL诱导的人脐静脉内皮细胞MCP-1 mRNA表达的影响  

Effect of Amlodipine on MCP-1 mRNA Expression in hUVEC-12 Induced by ox-LDL

作　　者：涂剑[1] 徐丽梅[2] 刘世远[3] 韦知樱[3] 涂玉林[2] 

机构地区：[1]南华大学药理教研室,湖南衡阳421001 [2]南华大学心血管病研究所 [3]南华大学

出　　处：《南华大学学报（医学版）》2009年第1期10-13,共4页Journal of Nanhua University(Medical Edition)

基　　金：湖南省医药卫生基金(B2007101);湖南省教育厅基金(07C635)资助课题

摘　　要：目的观察氨氯地平对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(hUVEC-12)单核细胞趋化蛋白1(MCP-1)mRNA表达的影响,以进一步探讨氨氯地平抗动脉粥样硬化的作用机制。方法筛选ox-LDL与hUVEC-12细胞孵育合适的处理浓度与时间,然后用不同浓度氨氯地平(0、0.1、1.0、10.0μmol/L)预孵育hUVEC-12后,与ox-LDL共同孵育,RT-PCR检测细胞MCP-1 mRNA表达的变化情况。结果筛选出ox-LDL与hUVEC-12共同孵育的合适浓度为50 mg/L,时间为24 h。氨氯地平可下调MCP-1 mR-NA的表达,并且对其表达的影响呈现浓度依赖性。结论氨氯地平可下调ox-LDL诱导的人脐静脉内皮细胞MCP-1 mRNA的表达。Objective To investigate the effects of amlodipine on the mRNA expression of monocytes chemotactic protein - 1 { MCP - 1 ） in human umbilical vein endothelial cell （ hUVEC ） by oxidized low density lipoprotein （ ox - LDL ）, so as to approach the mechanism of amlodipine in atherosclerosis. Methods hUVEC - 12 were treated with ox - LDL in different concentration or different time. Based on that, the cells were pretreated with amlodipine at different concentrations （ 0,0.1,1.0,10.0 μmol/L） and then incubated with ox - LDL, MCP - 1 mRNA expression was detected by reverse tran- scription - polymerase chain reaction （ RT - PCR）. Results MCP - 1 mB_NA expression induced by ox - LDL was obviously down - regulated by aralodipine in a dose - dependent manner. Conclusion Amlodipine may down - regulate the mRNA expression of MCP - 1 induced by ox - LDL in hUVEC - 12.

关 键 词：氨氯地平 单核细胞趋化蛋白1 氧化型低密度脂蛋白 人脐静脉内皮细胞 

分 类 号：R363[医药卫生—病理学]