4种提取鹰嘴豆基因组DNA方法的比较  

Comparison of Four Methods of DNA Extraction from Chickpea

作　　者：石庆华[1] 姚正培[1] 张桦[1] 许磊[1] 代培红[1] 

机构地区：[1]新疆农业大学农业生物技术实验室,乌鲁木齐830052

出　　处：《新疆农业大学学报》2009年第1期64-67,共4页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(30860152)

摘　　要：采用CTAB法、SDS-CTAB法、SDS法、尿素法4种方法对鹰嘴豆DNA进行提取,并从电泳结果、纯度、得率等方面对其进行比较研究,从而确定鹰嘴豆DNA提取适用流程。所提DNA纯度为:CTAB法>SDS-CTAB法>SDS法>尿素法;所提DNA的浓度(μg/mL)为:SDS-CTAB法>CTAB法>SDS法>尿素法。综合分析,CTAB法是提取鹰嘴豆DNA的最佳方法。The main object of this paper was to study DNA extracted from chickpea by the 4 methods ineluding CTBA method,SDS-CTAB method,SDS method and urea method and to carry out comparative analysis of the method and to carry out comparative analysis of the eletrophorctic results,purity and enzyme digesting of genome DNA. Thereby,the appropriate flow was determined for extracting DNA from chickpea. The purity of extracted DNA was as following：CTAB method〉SDS-CTAB method〉SDS method〉urea method. The concentration（μg/g） of extracted DNA was as following： SDS-CTAB method〉CTAB method〉SDS method〉urea method. The conclusion was drawn：CTAB method was the best one of extracting DNA from chickpea.

关 键 词：鹰嘴豆 CTAB法 SDS法 SDS—CTAB法 尿素法 

分 类 号：S529[农业科学—作物学]