重组人促红细胞生成素对压力作用下混合培养视网膜神经细胞凋亡及BCL-2表达的影响  

The Effects of Recombinant Human Erythropoietin on Apoptosis and BCL-2 Expression of Cultured Retinal Neurons under Pressure

作　　者：李珂[1] 张虹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第1期79-82,86,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的观察重组人促红细胞生成素(rhEPO)对压力作用所致鼠混合培养视网膜神经细胞凋亡的保护作用及可能的机制。方法将体外混合培养3 d的原代大鼠视网膜神经细胞随机分为对照组、单纯压力组和预处理压力组,预处理压力组分别加入0.15、0.30、0.50 U/ml rhEPO作用24 h后,单纯压力组和预处理压力组均在60 mmHg(1 mmHg=0.133 kPa)压力下培养24 h,然后TUNEL法检测细胞凋亡,免疫组化和RT-PCR法检测BCL-2蛋白质及mRNA的表达。结果单纯压力组细胞凋亡指数(AI)显著高于对照组(P<0.01);与单纯压力组比较,各不同浓度rhEPO预处理压力组的AI明显降低,同时BCL-2 mRNA及蛋白的表达均显著增加,差异均有显著性意义(P<0.05或P<0.01),且呈rhEPO浓度依赖性。结论rhEPO预处理可通过上调细胞凋亡抑制因子BCL-2的表达,从而抑制压力诱导的视网膜神经细胞的凋亡。Objective To study the protective effect of recombinant human erythropoietin （rhEPO） on the apoptosis of cultured retinal neurons induced by pressure and the possible mechanism. Methods The primary retinal neurons of postnatal SD rats were cultured in vitro for 3 days and divided into 3 groups： no pressure group, simple pressure group, and rhEPO pretreatment group. The retinal neurons in rhEPO pretreatment group were treated with 0.15, 0.30, or 0.50 U/ml rhEPO for 24 h. The cultured retinal neurons in simple pressure and rhEPO pretreatment groups were cultured under the pressure of 60 mmHg （1 mmHg=0. 133 kPa） for 24 h. The expression of BCL-2 mRNA and protein in retinal neurons was detected by RT-PCR and immunocytoehemistry, respectively. The apoptosis was assayed by TUNEL. Results The apoptosis index （AI） in simple pressure group was significantly higher than in no pressure group （P〈0.01）. As compared with simple pressure group, AI was significantly reduced, and the expression of BCL-2 mRNA and protein in rhEPO pretreatment groups markedly increased （P〈0.05 or P〈0.01） in a concentration-dependent manner. Conclusion The rhEPO pretreatment can inhibit the pressure-induced apoptosis of retinal neurons by up-regulating the expression of BCL-2.

关 键 词：重组人促红细胞生成素 视网膜神经节细胞 细胞凋亡 BCL-2 

分 类 号：R775[医药卫生—眼科]