山楂饴中苏丹红的高效液相色谱法测定  

Determination of SudanⅠand Ⅲ in Hawthorn Maltose by High Performance Liquid Chromatography

作　　者：周建科[1] 刘瑞英[1] 李晓阳[1] 

机构地区：[1]河北大学理化分析中心,河北省分析科学技术重点实验室,河北保定071002

出　　处：《现代食品科技》2009年第2期208-210,共3页Modern Food Science and Technology

摘　　要：采用反相高效液相色谱测定山楂饴中的苏丹红。样品经氯仿萃取后浓缩至干,乙醇定容。Diamonsil C18色谱柱,0.02%甲酸的甲醇溶液为流动相,检测波长500nm。在0.05～10.00mg/L范围内线性关系良好;苏丹红Ⅰ号、Ⅲ号的平均回收率分别为91.7%、93.4%,精密度分别为0.81%、0.96%;方法检出限为0.035μg/g;人工合成色素不干扰测定。A new method for determination of Sudan in hawthorn foods by reversed-phase high perfonnence liquid chromatography （RP-HPLC） was developed. The sample was extracted by chloroform, concentrated and then dissolved in alcohol. Using a Diamonsil C18 column, the pretreated samples were analyzed by a UV detector at 500 nm using a mixture of CHaOH and HCOOH（0.02%） as mobile phase at a flow rate of 1.0mL/min.Winthin the linear range of 0.05-10.00mg/L, the average recovery of sudan Ⅰ and Ⅲ by this method were 91.7% and 93.4%, respectively, and the precision of this method for sudan Ⅰ and Ⅲwere 0.81% and 0.96%, respectively. The detection limit of this method was 0.035μg/g. Artificial synthesized pigments in sample showed no disturbance for the determination of Sudan by this method.

关 键 词：苏丹红 高效液相色谱法 山楂饴 

分 类 号：TS207[轻工技术与工程—食品科学]