大豆7S球蛋白(α＋β)-亚基缺失型种质的α-与β-亚基基因的测序与分析  

Sequence Analysis of α- and β-Subunit Genes in a Novel Soybean Germplasm Characterized by Absence of α- and β-Subunit of 7S Globulin

作　　者：刘珊珊[1] 孟凡立[1] 刁桂珠[1] 王志坤[1] 葛玉君[1] 郑天慧[1] 姜自芹[1] 曾蕊[1] 李文滨[1] 

机构地区：[1]东北农业大学农学院大豆研究所,国家教育部大豆生物学重点实验室,哈尔滨150030

出　　处：《中国农业科学》2009年第2期419-424,共6页Scientia Agricultura Sinica

基　　金：哈尔滨市科技攻关计划项目(2008RFLXN002);东北农业大学引进人才项目;博士后研究人员落户黑龙江科研启动资助项目;第四十一批中国博士后科学基金资助项目(20070410885);国家863目标导向项目(2006AA10Z1F1);国家“十一五”科技支撑计划(2006BAD21B01)

摘　　要：【目的】明确大豆7S球蛋白(α+β)-亚基双缺失特性是否是由于α-与β-亚基基因的缺失或变异引起的。【方法】聚丙烯酰胺凝胶电泳(SDS-PAGE)分析和α-与β-亚基基因的PCR扩增、克隆、测序和序列比较。【结果】该种质α-亚基基因特异性引物的PCR扩增结果与对照基本一致,β-亚基基因的则与对照不同。α-亚基基因扩增片段与对照的同源性较高,为90.9%,二者的区别主要存在于扩增片段的5′-端;β-亚基基因间的同源性较低,仅为53.1%。另外,在该材料β-亚基基因扩增片段的两端,检测到一对短的反向重复序列。【结论】该种质α-与β-亚基同时缺失的特性不是由α-与β-亚基基因的缺失引起的;α-与β-亚基基因的扩增序列与对照存在一定的差异,基因序列间的差异是否为导致α-与β-亚基缺失的直接原因,尚需进一步研究确认。[ Objective ] The objective of this study was to elucidate whether the α- and β- null character was result from the absence or variance of α- and β-subunit genes. [Method] SDS-PAGE technique, PCR amplification, cloning and sequencing methods were used. [Result] With specific primers for α- subunit, the PCR band pattern between （α＋β）-null type and normal type was identical, but the PCR band pattern of β-subunit gene was unique. For PCR amplified DNA fragment of the α-subunit gene, a high degree of nucleotide homology （90.9%） exists between the （α＋β）-null type and normal type. Sequenced difference mainly exists in 5′-flanking. While, for fl-subunit gene, a low degree of nucleotide homology （53.1%） was exists. Furthermore, a short inverted sequence was detected in PCR amplified DNA fragment of the β-subunit gene.[ Conclusion ]The deficiency character of α- and β-subunit of （α＋β）-null type is not related to the deletion of a chromosome segment with the genes encoding α- and β-subunit of soybean 7S globulin. Differences exist in both α- and β-subunit gene sequence between （α＋β）-null type and normal type soybean. Further studies to confirm whether the differences in gene sequence are main reason that result in the absence of α- and β-subunit are necessary.

关 键 词：大豆 7S球蛋白 α-与β-亚基 稀有亚基组合 DNA测序 

分 类 号：S565.1[农业科学—作物学]