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作 者:柳小春[1] 肖调义[1] 何伟光[1] 马海明[1] 刘鑫[1] 刘峰[1] 何俊[1]
出 处:《中国农业科学》2009年第2期742-747,共6页Scientia Agricultura Sinica
基 金:湖南省科技厅重点攻关项目(04NK2005)
摘 要:【目的】研究猪生长性状相关基因多态性,各多态位点对猪生长及胴体性状的影响,为分子标记辅助选择提供依据。【方法】采用PCR-RFLP方法分析PIT1、HDAC1、IGF2基因在不同品种酶切位点多态性,分析不同基因型对生长与胴体性状的遗传效应。【结果】PIT1基因C等位基因、HDAC1基因G等位基因、IGF2基因A等位基因均有显著提高生长的效应,达100kg活重所需时间一般可缩短6~9d。PIT1基因C等位基因、HDAC1基因G等位基因、IGF2基因A等位基因提高瘦肉率3~5个百分点、背膘厚降低2~3mm,生长性能指数得到相应提高。【结论】上述基因的有利等位基因有可能在选种中作为分子标记。[Objective] The objective of this study was to study the polymorphism of porcine three loci of PIT1, HDAC1 and IGF2 and its relationship with the growth and carcass traits in swine. [ Method ] The polymorphism of three genes was analyzed by PCR-RFLP method. The genotypic effects of such genes on growth and carcass traits were analyzed. [Result] CC genotype of PIT1, GG of HDAC1 and AA of IGF2 had favorable effect on growth and the age at 100 kg liveweight is 6-9days less than the contrary genotypes. The effects of CC genotype of PIT1, the GG genotype of HDAC1 and the BB genotype of IGF2 on lean percentage were 3-5 percent higher and on backfat thickness were 2-3 mm lower than the contrary genotypes. The index of growth traits increased relevantly. [Conclusion] The favorable genotypes of thoese genes could be regarded as molecular markers in selection of lean type pigs.
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