抗甲型流感病毒血凝素通用抗体的制备及鉴定  

Establishment and identification of universal antibody against all subtypes of the influenza A viral hemagglutinins

作　　者：李长黄[1] 李娟[1] Stella Chun 邵铭[1] 刘书珍[1] 崔晓雨[1] 黎旭光[2,3] 方捍华[1] 王军志[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]加拿大渥太华大学生化系 [3]加拿大卫生部生物制品研究中心

出　　处：《中华微生物学和免疫学杂志》2009年第2期126-129,共4页Chinese Journal of Microbiology and Immunology

基　　金：科技部“十五”国家科技攻关计划（2004BA519A69）

摘　　要：目的建立针对甲型流感病毒血凝素的通用抗体。方法通过对流感病毒数据库中血凝素基因的分析，寻找保守的序列并合成多肽。将多肽耦联在载体匙孔血蓝蛋白（KLH）上，多次皮下免疫家兔，制备高效价抗体。结果序列分析发现血凝素第二亚单位（HA2）上氨基端14个氨基酸极端保守，耦联多肽经4次免疫后效价达到1：80000，以免疫印迹方法证明此通用抗体能与流感病毒H1～H13亚型的血凝素蛋白特异反应。结论通过寻找保守蛋白并耦联在合适载体上，免疫家兔获得了针对甲型流感病毒血凝素抗原的通用抗体。Objective To establish the universal antibody against all subtypes of the influenza A viral hemagglutinins（HA）. Methods All HA sequences from influenza viruses in common domains were analyzed to search for the most conservative region of the HA gene. The peptides conjugated to carrier proteins were used to immunize rabbits in order to obtain high titer antibody. Results The most conservative region among all influenza viruses HA genes were 14 amino acids located at the N-terminal of HA2. The antibody titer reached to 1 ： 80 000 after 4 injections of the coupled peptides. The achieved antibody was demonstrated by Western blot to bind HA proteins of influenza virus subtypes H1-H13 specifically. Conclusion The universal antibody has been successfully established by immunizing the rabbits with most conservative peptides of HA protein coupled to cartier protein.

关 键 词：甲型流感病毒 血凝素 通用抗体 免疫印迹 

分 类 号：R512.39[医药卫生—内科学] R392.11[医药卫生—临床医学]