中国HIV-1 B′/C亚型感染者对异体病毒中和作用与疾病进展关系研究  

Study on the relationships between neutralizing antibody response against heterologous virus and dis- ease progression of HIV type 1 B′/C infected individuals in China

作　　者：包名家[1,2] 耿文清[1] 崔华露[1] 张晓丽[1] 徐东兵[1] 王亚婷[1] 潘莹[1] 姜拥军[1] 王亚男[1] 韩晓旭[1] 张旻[1] 张子宁[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001 [2]黑龙江省佳木斯市疾病预防控制中心,154002

出　　处：《中华微生物学和免疫学杂志》2009年第2期165-169,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项课题资助项目（2008zx10001-001）;国家重点基础研究发展计划973计划（2006CB504206）;辽宁省教育厅高等学校创新团队项目（2006T134）

摘　　要：目的探讨中国HIV-1 B′／C亚型感染者对异体病毒中和作用与疾病进展的关系。方法根据CD4T淋巴细胞数量和有无临床症状将HIV-1 B′／C亚型感染者分为HIV慢性感染组和AIDS组。将HIV-1感染者血清稀释（1／10～1／320）后，与在基因结构特点上同源性很低的3株HIV-1作用，以检测其中和作用。同时以正常人血清加病毒悬液为对照孔，能够抑制对照孔50％病毒复制的血清为中和作用阳性。将某个HIV-1感染者血浆能够中和异体病毒的个数占3个异体病毒的百分率定义为HIV-1感染者中和异体病毒的宽度；将某个HIV-1感染者血浆中和3个异体病毒抗体滴度的几何平均滴度定义为HIV-1感染者中和异体病毒的强度。结果HIV-1慢性感染组与AIDS组之间中和异体病毒的宽度和强度差异有统计学意义，HIV-1慢性感染组显著高于AIDS组。HIV-1慢性感染组中和异体病毒的宽度和强度与病毒载量呈正相关，而AIDS组中和异体病毒的宽度和强度与病毒载量没有显著的相关性。HIV-1慢性感染组和AIDS组中和异体病毒的宽度和强度与CD4T淋巴细胞数均没有显著的相关性。结论中国HIV-1B′／C亚型感染者不同疾病进展阶段针对异体病毒中和作用能力不同，HIV慢性感染组显著高于AIDS组，当疾病进展到AIDS期时，失去对异体病毒的中和作用，提示针对异体病毒的中和抗体与疾病进程有关。Objective To study the relationships between neutralizing antibody response against heterologous virus and disease progression in Chinese HIV-1 B′/C infected individuals. Methods Plasmas from HIV-1-infected individuals, grouped as HIV chronically infected or AIDS according to CD4^＋ count and clinical symptom, were tested for neutralizing activity against the three HIV-1 isolates with very low homology in vitro. Six two-fold dilutions of each plasma sample （from 1/10 to 1/320） were tested against each virus from the panel. Giving a 50% reduction in p24Ag compared with normal human plasma control wells was defined as positive. The breadth of the cross-neutralizing response was defined based on the number of viruses that were effectively neutralized by any given patient-derived plasma sample. The magnitude of the crossneutralizing response was defined based on the average neutralizing titer against all heterologous viruses. Resuits We found that there revealed a significant difference between HIV chronically infected and AIDS group in the breaths and magnitudes of neutralizing heterologous virus. There was higher prevalence for the frequency of neutralizing heterologous virus in HIV chronically infected than AIDS. The results showed that there was positive correlation between the breadths and magnitudes of neutralizing response against heterologous virus and the plasma HIV RNA level in HIV chronically infected group, while not in AIDS group. There was no association between the breadth of the neutralizing responses against heterologous virus and CD4 T cell counts. Conclusion The capacity of neutralizing antibodies against heterologous virus varied among different disease stage. There were higher titers of neutralizing antibodies in HIV chronically infected than AIDS group. The loss of neutralizing antibodies in plasma from AIDS group appears to be associated with a narrowing of the antibody response during disease progression. These suggest that the presence of neutralizing antibodies against hetreologous vi

关 键 词：HIV-1 B′/C亚型 中和抗体 异体病毒 

分 类 号：R51[医药卫生—内科学]