黄海北部不同站位海洋细菌群落分布特征  

Bacterial distribution at different stations in the Northern Yellow Sea

作　　者：白洁[1] 李海艳[1] 赵阳国[1] 

机构地区：[1]中国海洋大学环境科学与工程学院,青岛266100

出　　处：《微生物学报》2009年第3期343-350,共8页Acta Microbiologica Sinica

基　　金：国家908专项基金(908-01-ST02);国家自然科学基金(40801193)~~

摘　　要：【目的】为揭示北黄海不同海域中真细菌群落分布的差异,【方法】采用16SrRNA基因文库和变性梯度凝胶电泳(DGGE)技术,对远海和近海两个站位的沉积物和水体中细菌群落特征进行了解析和评价。【结果】文库分析揭示海水及沉积物中微生物种类丰富,存在大量未被认知的类群。各站位中主要为变形菌门(Proteobacteria),沉积物中γ-Proteobacteria和δ-Proteobacteria亚门占优势,水中则以α-Proteobacteria亚门占优势,但各亚门微生物在两个站位中存在明显系统发育学分歧。DGGE图谱聚类分析显示,近海沉积物和海水中细菌群落优势类群相似性很高,而远海沉积物和海水中则相似性很低。【结论】研究结果表明,微生物种类在不同地理位置和生存介质中存在明显差异,环境因素对微生物的分布起主导作用。[Objective] To reveal the diversity of marine bacteria in disparate sites of the Northern Yeallow Sea. [Methods] Bacterial community structure and diversity within seawaters and sediments at two stations in the Northern Yellow Sea were investigated and assessed by the 16S rDNA clone library and the denaturing gradient gel electrophoresis （DGGE） techniques.[Results] The 16S rDNA clone library analysis indicated that the bacteria were abundant in the waters and sediments, and most of them were unknown. Phylogenetic analysis showed that the Proteobacteria were the dominant group both in the sediments and waters, γ-Pmteobacteria and β-Pmteobacteria were dominant in the sediments, α-proteobacteria were dominant in the water. However, all subphyla of Proteobacteria presented phylogenetical divergence at the two sites. The clustering analysis on the DGGE patterns revealed that the dominant groups within the waters and sediments in shore were similar to each other, whereas that in the offshore were different.[Conclusion] The microbial diversity differed due to the geographical location and living medium; and the microbial distribution depended on the environment factors.

关 键 词：北黄海 16S RRNA 变性梯度凝胶电泳(DGGE) 变形菌门 

分 类 号：Q938[生物学—微生物学]