破骨细胞对多孔碳酸钙陶瓷材料物降解的实验研究  

Biodegradation Effects of Osteoclasts on Porous Calcium Carbonate Ceramics in Vitro

作　　者：杨帆[1] 谭金海[1] 邢林卿[1] 熊亮[1] 雷鹏程[1] 

机构地区：[1]武汉大学中南医院骨科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2009年第2期159-162,F0002,共5页Medical Journal of Wuhan University

基　　金：湖北省科技攻关项目(编号:2005AA301C17)

摘　　要：目的:采用破骨细胞与多孔碳酸钙陶瓷(PCCC)体外混合培养方法,观察其对PCCC的降解作用。方法:从新生SD大鼠长骨骨髓中分离出破骨细胞,与PCCC材料混合培养,分别于48h及96h终止培养并观察。结果:在倒置显微镜下见破骨细胞体呈圆形或椭圆形,多核,胞质伸出许多细微突起。抗酒石酸酸性磷酸酶(TRAP)染色阳性。扫描式电子显微镜观察见在PCCC表面出现许多散在分布的吸收空隙,直径一般50μm,破骨细胞位于其中。96h见吸收空隙增大。结论:破骨细胞在体外培养条件下对PCCC材料有明显吸收降解作用。Objective： To observe the biodegradation of porous calcium carbonate ceramics （PCCC） through mixed culture of osteoclasts and PCCC in vitro. Methods： Osteoclasts were isolated from neonatal SD rats＇ marrow of long bone and cultured on PCCC. The culturing was ended separately at 48 h and 96 h. Results： Under inverted microscope, the osteoclasts presented round or oval bodies with multi-nuclei and many thin projections. These cells were stained positively with tartaric acid resistance acid phosphatase （TRAP）. Scanning electron microscope showed that many resorption lacunae were generated on PCCC surface with the diameter less than 50 μm. Osteoclasts were located in the lacunae. At 96 h, the resorption lacunae become bigger. Conclusion： Osteoclasts showed resorption ability to PCCC in vitro.

关 键 词：降解 破骨细胞 多孔碳酸钙陶瓷 

分 类 号：R783.1[医药卫生—口腔医学]