盐酸壳聚糖对兔角膜上皮损伤的修复作用  

Promotive Effects of CH-HCL Chitosan Solution on Epithelial Corneal Abrasion in Rabbits

作　　者：徐永红[1] 邢怡桥[1] 张少维[1] 陈效[2] 伍志琴[1] 陈曦[1] 

机构地区：[1]武汉大学人民医院眼科,湖北武汉430060 [2]华中科技大学同济医学院药理学教研室,湖北武汉430030

出　　处：《武汉大学学报（医学版）》2009年第2期173-176,I0004,共5页Medical Journal of Wuhan University

摘　　要：目的:探讨不同浓度的盐酸壳聚糖眼液对兔角膜上皮损伤的修复作用。方法:将42只新西兰大白兔随机分成5组,其中2只制作角膜上皮损伤模型后裂隙灯荧光素染色照相后处死,其余40只(每组10只),分别为A组:0.5%盐酸壳聚糖组;B组:1.5%盐酸壳聚糖组;C组:贝复舒组(阳性对照组);D组:生理盐水组(阴性对照组)。将4组兔双眼角膜中央置入直径7.00mm的环钻,其内滴入20%乙醇约60s后用角膜刮匙刮去角膜上皮,制作角膜上皮缺损模型,术后每组滴用眼液频率均为3次/d,各组分别于术后12,24,36,48及72h行裂隙灯下荧光素染色照相及海德堡共焦激光角膜显微镜检查后各处死2只兔,取角膜做病理切片同时应用计算机图像处理系统测量角膜损伤后愈合面积。结果:A,B,C,D各组角膜上皮愈合速率分别为17.22,23.63,22.53,11.16mm2/d。B组及C组作用稍强,但BC组间差异无显著性,且4组均未见显著的炎症反应及眼球刺激症状。结论:0.5%及1.5%盐酸壳聚糖眼液对角膜上皮损伤的愈合有明显的促进作用,且无副作用。Objective： To investigate the promotive effects of different concentration of CH-HCL chitosan solution on the epithelial corneal abrasion in rabbits. Methods： A total of 42 rabbits were randomly divided into five groups. Two rabbits were set as epithelial corneal abrasion models at baseline （killed at 0 h）, the other four groups （10 rabbits in each group） were treated respectively with 0.5%CH-HCl （group A）, 1.5%CH-HCl（group B）, recombinant hunman （bovine） basic fibroblasts （group C, positive control group）, and normal saline （group D, negative control group）. A corneal abrasion with a diameter of 7 mm was created centrally in both eyes with a trephine and subsequent rubbing off on the corneal epithelium with a 20% alcohol soaked swab. Photographic documentation and hematoxylin and eosin stained pathological section were performed when rabbits were killed at 0, 12, 24, 36, 48 and 72 h. Planimetry was performed, and the corneal photographs were analyzed with computer software. Results： The healing rate of A to D groups was 17.22, 23.63, 22.53, and 11.16 mm^2/d respectively. The group B and C recov ered much sooner than groups A and C did. There were no side effects observed in all the rabbits＇ eyes. Conclusion： CH-HCl has marked facilitation effects in the healing course of rabbit＇s epithelial corneal.

关 键 词：盐酸壳聚糖 角膜上皮 愈合 

分 类 号：R772.2[医药卫生—眼科]