环孢素A对人滋养细胞nm23-H1表达的调控作用  

Regulation of cyclosporin A on nm23-H1 expression in human trophoblast

作　　者：侯晓帆[1] 谢可鸣[1] 李明清[2] 李大金[2] 

机构地区：[1]苏州大学医学部病理生理学教研室,江苏苏州215123 [2]复旦大学上海医学院附属妇产科医院,妇产科研究所,上海200011

出　　处：《中国病理生理杂志》2009年第3期493-496,共4页Chinese Journal of Pathophysiology

基　　金：国家重大科学研究计划资助项目(No.2006CB944009);国家自然科学基金资助项目(No.30670787);上海市基础研究重点资助项目(No.03JC14016);上海市医学领军人才专项研究基金资助

摘　　要：目的:探讨环孢素A(CsA)对人滋养细胞系Bewo的23号非转移性基因(nometastatic gene 23)H1型即nm23-H1表达的调控作用,为治疗滋养细胞疾病提供新的依据。方法:将Bewo细胞分为对照组(加溶媒)、环孢素组加入终浓度由10-2μmol/L-10μmol/L环孢素A。分别于48h后用RT-PCR检测nm23-H1基因mR-NA表达水平,于72h后用Incell Western分析nm23-H1蛋白表达水平。结果:与对照组相比,nm23-H1的表达水平随环孢素A浓度10-2μmol/L-1μmol/L呈现降低趋势,其中1.0μmol/L环孢素Anm23-H1 mRNA和蛋白水平均明显降低(P<0.05,P<0.01);当浓度升高至10μmol/L时,nm23-H1则呈升高趋势。结论:低浓度环孢素A可通过降调节nm23-H1的表达,继而改善滋养细胞的侵袭力。AIM ： To explore whether cyclosporine A （CsA） can regulate the expression of nometastatic gene23 H1 type （ nm23 - H1 ） in human choriocarcinoma Bewo cells, in order to seek new proof of treating trophoblast diseases. METHODS： The Bewo cells were divided into two groups. The vehicle control group, and the CsA group with different concentrations from 10 -2 μmol/L to 10 μmol/L. The effect of CsA on the transcription of nm23 - H1 was determined by reverse transcription - polymcrase chain reaction （ RT - PCR） after cultured for 48 h and protein level of nm23 - H1 was determined by In - cell Western after cultured for 72 h. RESULTS ： Compared to the vehicle group, CsA significantly down- regulated the transcription and translation of nm23 -H1 in a dose -dependent manner from 10-2 μmol/L to 10 μmol/L, and the inhibition reached its top when concentration of CsA was 1.0μmol/L （P 〈 0. 05, P 〈 0. 01 ）. However, when concentration of CsA rised to 10 μmol/L, the expression of rim23 -H1 increased significantly. CONCLUSION： The result above indicates that CsA at an appropriate lower concentration can inhibit the expression of the nm23 - H1, which involves in invasiveness of human trophoblast cells.

关 键 词：环孢菌素 NM23-H1 滋养细胞 

分 类 号：R363[医药卫生—病理学]