CIK/NK细胞4周培养过程中的CD27、CD28的表达及其意义  

Changes of the expression of CD27 and CD28 on cytokine-induced killer cells and natural killer cells during four week cultivation

作　　者：吴燕峰[1] 黎阳[2] 王潇娉[2] 方建培[2] 魏菁[3] 

机构地区：[1]中山大学附属第二医院干细胞研究中心,广东广州510120 [2]中山大学附属第二医院儿科,广东广州510120 [3]中山大学附属第二医院医学研究中心,广东广州510120

出　　处：《中国病理生理杂志》2009年第3期525-528,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30500469);广州科学仪器协作共用网基金资助项目(No.2006034)

摘　　要：目的:了解细胞因子诱导杀伤(CIK)细胞和自然杀伤(NK)细胞在培养过程中CD27和CD28的表达变化规律。方法:通过流式细胞仪检测CD3+CD56+CIK细胞以及CD3-CD56+NK细胞在4周培养过程中其上CD27和CD28的表达变化。结果:CD27在CIK和NK细胞上均于培养的第2周达到最高峰,分别为(44.57±4.07)%和(51.02±5.20)%,但之后逐步下降,在培养第4周降至30%左右的水平。CD28在CIK和NK细胞上分别是在第3周和第2周时达到表达的最高峰,为(4.40±0.66)%和(45.14±3.58)%,之后迅速下降,在培养第4周已经几乎消失。结论:根据CD27和CD28在CIK/NK细胞上的表达变化规律,CIK/NK细胞的收获时机以培养第3周为宜。AIM： To investigate the changes of CD27 and CD28 expression on cytokine -induced killer（CIK） cells and natural killer（NK） cells during cultivation. METHODS： The mononuclear cells were treated with interleukin- 12（ IL-2） ,IL- 7, IL- 15, stem cell factor and FLT3 ligand for four weeks. The CD27 and CD28 expression on CD3^＋ CD56 ^＋ CIK cells and CD3 - CD56 ＋ NK cells were examined by flow cytometric analysis every week during four - week cultivation. RESULTS： The expression of CD27 on CIK and NK cells two weeks after cultivation reached the peak value at （44. 57±4. 07）% and（51.02 ±5.20）%, respectively. Then, their expression declined gradually to about 30% in the fourth week. The expression of CD28 on CIK and NK cells reached the peak at the third and the second week, the values were （4.40±0.66）% and （45.14 ± 3.58 ）%, respectively, decreased rapidly and then were almost completely lost at the fourth week. CONCLUSION： According to the changes of expressions of CD27 and CD28 on CIK/NK ceils, optimal harvest time of cultured CIK/NK cells should be at the third week of cultivation.

关 键 词：细胞因子诱导杀伤细胞 自然杀伤细胞 CD27 CD28 过继免疫治疗 

分 类 号：R73-362[医药卫生—肿瘤]