一株解磷中度嗜盐菌的分离鉴定及解磷特性分析  

Identification of a Moderate Halophilic Phosphate-dissolving Bacterium and Its Phosphate-solubility

作　　者：向文良[1,2] 冯玮[1] 郭建华[3] 宋鹏[1] 汤科[1] 杨志荣[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064 [2]西华大学生物工程学院,四川成都610039 [3]西南大学荣昌校区动物医学系,四川荣昌402460

出　　处：《微生物学通报》2009年第3期320-327,共8页Microbiology China

基　　金：“十一五”国家科技支撑项目(No.2007BAC18B03)

摘　　要：从四川自贡某盐井壁植物根系土壤中分离得到1株中度嗜盐解磷菌QW1011。该菌细胞呈线状,大小为0.8μm×30μm～100μm,革兰氏染色为阳性、最适NaCl生长浓度为10%,NaCl最高耐受浓度15%。好氧生长,酪素水解、硝酸还原和接触酶阴性。菌株的16S rRNA基因序列(接受号:EF647207)与Bacillus megatherium ATCC14581的16S rRNA相似性为100%,其16S-23S rRNA间区(ISR)的PCR扩增片的PAGE指纹图谱与参考菌株Bacillus megaterium ATCC14581和Bacillus megatherium var.phosphaticum DSM3228存在较大的差异。基于形态学、生理生化、16S rRNA及ISR的初步分析,认为中度嗜盐解磷菌QW1011为Bacillus属,对其系统分类还需结合其它分类学指标做进一步分析。当温度24°C～36°C,NaCl浓度5%～10%,pH6.5～8.0培养60h时,菌株QW1011对Ca3(PO4)2盐培养基都具有较好的解磷能力,其解磷能力可达56.76μg/mL～71.34μg/mL,且对Ca3(PO4)2盐的解磷能力优于卵磷脂的解磷能力。A aerobic moderate halophilic phosphate-dissolving bacterium, named QW1011, was isolated from the plant rhizospheres grown on the wall of ancient brine well located in Zigong city, Sichuan Province, China. The results showed that its cell was line shaped （0.8 μm×30μm-100 μm）, gram positive. The strain required aerobic conditions for growth and grew well with an optimum concentration at 10% NaCl （W/V） at pH 7.0 and tolerated up to 15% NaCl. And it could also hydrolyze gelatin and starch but not casein. 16S rRNA analysis revealed that its sequence sharing 100% similarity to B. Megaterium ATCC 14581. However, compared with B. Megaterium ATCC 14581 and B. Megatherium var. phosphaticum DSM 3228, QW1011 exhibited several differences in the phenotypic, physiological characteristics and ISR. Based on the phenotypic distinctiveness, molecular and ISR genetic evidence, it was proposed that QW1011 can be classified as genus Bacillus. The strain showed high phosphate-dissolving ability for Ca3（PO4）2 with the capacity of 62.42 μg/mL-71.34μg/mL in Ca3（PO4）2 liquid medium at 24℃-36℃, 5%-10% sodium （W/V） and pH 6.5-8.0 after incubation for 60 h. And its activity for Ca3（PO4）2 was higher than for lecithin.

关 键 词：中度嗜盐解磷菌 解磷分析 表型分析 16S rRNA系统发育 16S-23S rRNA间区特性 

分 类 号：S154.3[农业科学—土壤学]