人凝血因子C同源物基因全长cDNA克隆  

Full Length cDNA Cloning of Human Coagulation Factor C Homology

作　　者：孙勍[1] 程静[2] 王国建[2] 刘新[2] 于飞[2] 韩冰[2] 陈静[2] 戴朴[2] 袁慧军[2] 韩东一[2] 

机构地区：[1]武警总医院耳鼻咽喉头颈外科,北京100039 [2]解放军总医院耳鼻咽喉科研究所

出　　处：《听力学及言语疾病杂志》2009年第2期165-168,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金面上项目(30371523);国家自然科学基金面上项目(30571018);国家自然科学基金海外青年学者合作基金(30528025)资助

摘　　要：目的通过克隆凝血因子C同源物基因(Coagulation factor C homology,COCH)全长cDNA,为COCH编码蛋白cochlin的功能研究打下基础。方法从听力正常人新鲜外周静脉血中提取总RNA,应用一步法RT-PCR试剂盒进行COCH反转录,转录产物进行浓缩、酶切、连接。结果反转录COCH cDNA全长与标准基因序列比较,结果完全相符,得到完整的COCH cDNA全长序列。结论本研究成功克隆了COCH cDNA全长序列,为COCH编码蛋白cochlin的功能研究打下了良好的基础。Objective To obtain full--length cDNA of human coagulation factor C homology （COCH） gene. Methods Total RNAs were extracted from the peripheral venous blood. One step RT--PCR kit was used to carry out reverse transcription. The transcript was condensed, digeseted by Hind Ⅲ restriction enzyme and ligated by DNA ligation kit. Results Comparing with standard gene order from gene bank, the acquired full--length eDNA sequence was coincident eompletely. Conclusion In our studies, the full--length eDNA of human coagulation factor C homology was cloned successfully, it will facilitate our future studies on functions of coehlin protein.

关 键 词：克隆 凝血因子C同源物基因 互补DNA 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]