应用巢式PCR检测猪戊型肝炎病毒核酸及其序列分析  

Nested PCR to detect nucleic acid of swine hepatitis E virus and sequence analysis

作　　者：邱蜜蜜[1] 丛彦龙[1] 丁壮[1] 孟轲音[2] 李志杰[1] 尹仁福[1] 李少丽[1] 王昌庆[1] 刘美[1] 吴昊[1] 母连志[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2009年第3期296-298,307,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30771606)

摘　　要：根据GenBank注册的AJ272108等序列,利用Oligo6应用软件设计内外2对引物,采用RT-PCR技术对本实验室分离的猪戊型肝炎病毒进行探索性扩增,并对测定的序列进行分析,结果用套式引物扩增出了目的条带,序列分析表明该序列属于基因Ⅳ型,并与其核苷酸同源性在85.6%～94.8%之间,氨基酸同源性在99.1%～100%之间。而与基因Ⅲ型核苷酸同源性最低,与基因Ⅱ型氨基酸同源性最低。According to GenBank registered AJ272108 sequence, etc, and referring the literatures of Meng and other reSearchers,then using Oligo6 software to design two pairs of primers, using RT-PCR technology to amplificate swine hepatitis E virus which was isolated from the laboratory and analysising the sequence. The results showed that using Nested primers to expand the fragment,and sequence analysis showed that the gene sequence belong to genotypes 4,and with genotypes 4 between 85.6% and 94.8% in nucleotide identity,between 99.1% and 100% in the amino acid identity. But keeping the minimum with genotypes 3 in nucleotide identity,and keeping the minimum with genotypes 2 in amino acid identity.

关 键 词：猪戊型肝炎病毒 巢式PCR 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]