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作 者:单立莉[1] 张敏[1] 苗志国[2] 王雷杰[3] 许梓荣[3]
机构地区:[1]延边大学农学院动物科学系,吉林龙井133400 [2]河南科技学院动物科技学院,河南新乡453003 [3]浙江大学动物科学学院饲料研究所,浙江杭州310029
出 处:《中国兽医学报》2009年第3期374-377,388,共5页Chinese Journal of Veterinary Science
摘 要:运用半定量RT-PCR方法对不同生长阶段金华猪和长白猪背最长肌中肌细胞生成素(Myogenin,MyoG)基因mRNA的表达丰度进行测定,分析与肌肉沉积的关系。结果表明:在2种猪35、80、125日龄均可检测到MyoG mRNA,金华猪背最长肌中MyoG基因的表达随着年龄和胴体瘦肉率的增加而增加,并且MyoG基因表达与胴体瘦肉率呈正比;而长白猪背最长肌中MyoG基因的表达恰好相反。除35日龄以外品种间无显著差异。Myogenin (Myogenin,MyoG) mRNA abundance in Longissimus dorsi muscle (LDM) of Jinhua pigs and Landrace pigs (35,80,125 days of age) were determined by semi-quantitative RT-PCR. The results showed that:during the whole expreimental period,the expression of MyoG gene increased with the age and muscle percentage of carcass in LDM of jinhua pigs,the expression levels of MyoG gene and muscle percentage of carcass were directly correlated. In contrast, the expression of MyoG gene decreased with the age and muscle percentage of carcass in Labdrace pigs,the expression levels of MyoG gene and muscle percentage of carcass were negatively correlated, but did not display breed differences(other than the 35 day-old outside).
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