恩诺沙星单克隆抗体的制备及酶联免疫分析方法的建立  

Production of Monoclonal Antibodies Against Enrofloxacin and the Establishment of Enzyme-linked Immunosorbent Assay

作　　者：冯婷婷[1] 刘一兵[1] 李子颖[1] 贾娟娟[1] 袁志刚[1] 韩世泉[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《同位素》2009年第1期5-9,共5页Journal of Isotopes

摘　　要：采用抗恩诺沙星单克隆抗体建立酶联免疫吸附法(ELISA)用以检测恩诺沙星在动物源性食品中的残留。经方法学鉴定,本方法的检测范围为0.5～50μg/L,灵敏度为0.2μg/L,批内变异系数<10%,批间变异系数<20%。鸡肉、鱼肉、虾和蜂蜜样品的回收率分别为95.5%～107.5%、80.0%～101.3%、105.7%～122.7%和93.3%～111.3%。方法学鉴定结果符合免疫分析的基本要求。An enzyme-linked immunosorbent assay （ELISA） using monoclonal antibodies against enrofloxacin （ENR） was developed to analyzed ENR residues in animal-based food products. The standard line of the assay was in the rang of 0.5-50μg/L. The sensitivity was 0.2μg/L. The intra- and inter-assay CVs of 3 samples were lower than 10% and 20% respectively. The recoveries of chicken muscle tissues, fish, shrimp and honey were in the range of 95. 5%-107. 5%,80. 0%-101. 3%, 105. 7%-122. 7% and 93. 3%-111.3% respectively.

关 键 词：恩诺沙星(ENR) 单克隆抗体 酶联免疫分析 

分 类 号：R446.61[医药卫生—诊断学]