大鼠白蛋白酶联免疫分析试剂盒的研制  

Development of ELISA Kit for Rat Albumin

作　　者：袁志刚[1] 韩世泉[1] 刘一兵[1] 许文革[1] 贾娟娟[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《同位素》2009年第1期23-27,共5页Journal of Isotopes

摘　　要：采用大鼠白蛋白免疫绵羊制得羊抗大鼠白蛋白抗血清,建立了大鼠白蛋白酶联免疫分析方法。本方法的测量范围为1～50 mg/L,灵敏度为0.42 mg/L,回收率为85.0%～106.0%,批内、批间变异系数分别<8.9%和<12.8%,高浓度大鼠尿样系列倍比稀释后测定,实测值和计算值呈线形相关,相关系数r=0.999。本方法学与放射免疫分析(RIA)方法学的相关性方程为y=0.92x+0.08,r=0.976,两种方法的相关性良好。方法学鉴定结果符合免疫分析的基本要求,大鼠白蛋白酶联免疫试剂盒的研制为肾病药物的开发及肾损伤的研究提供了方便。The Anti-rat albumin serum was prepared by immunized the sheep with rat albumin. A ELISA method was established for rat albumin. The measurement range of the assay was 1-50 mg/L, sensitivity of the assay was 0. 42mg/L, recovery rate was 85.0%- 106.0%. Intra- and inter-assay variation coefficients were 〈8.9 % and 〈12.8% respectively. The correlation coefficients between measured and expected values were 0. 999 after serial dilution of the urine samples with high concentrations of rat albumin. A good correlation was observed between the ELISA and RIA methods, and the kit for rat albumin might provide a convenience in exploitation of renal drugs and experimental injury of the kidney.

关 键 词：酶联免疫分析 大鼠白蛋白 放射免疫分析 肾损伤 

分 类 号：R446.61[医药卫生—诊断学]