中华眼镜蛇毒膜毒素对人类膀胱癌细胞EJ-m3作用的体外研究  

Role in the mechanism high - invasiveness of human bladder cancer cell line EJ - m3 treated by membrane toxin from naja naja atra venom in vitro

作　　者：何汇海[1] 杨德林[1] 王婉瑜[1] 谢蜀生[1] 王剑松[1] 徐鸿毅[1] 

机构地区：[1]昆明医学院第二附属医院泌尿外科,云南650101

出　　处：《国际泌尿系统杂志》2009年第2期171-174,共4页International Journal of Urology and Nephrology

基　　金：昆明医学院研究生创新基金资助项目（KM2007L12）

摘　　要：目的研究中华眼镜蛇毒膜毒素（MT-12）对人膀胱癌细胞株EJ-m3的作用，并探讨其作用机制。方法以穿透人工基底膜能力为依据筛选具有体外高侵袭能力的膀胱癌细胞；采用四氮唑蓝（MTT）法检测MT-12不同浓度对EJ-m3细胞的生长抑制率；同时流式细胞仪检测不同浓度MT-12作用24h后细胞中趋化因子受体CXCR4的表达。结果经过反复筛选获得膀胱癌细胞EJ的体外高侵袭力亚系，EJ-m3。MT-12可有效抑制EJ-m3细胞的生长，具有浓度依赖性特点。MT-12对EJ-m3作用72h的IC50是0．66ug／mL；流式细胞仪检测结果显示，在0．125～0．5ug／mL，MT-12组随着药物浓度的增加CX-CR4的表达逐渐减弱（P=0．020）。结论MT-12可有效抑制膀胱癌细胞生长，其作用机制可能与CXCR4在膀胱癌细胞中高表达有关。MT-12有潜力成为新的抗膀胱癌药物。Objectives To investigate the effects of the membrane toxin 12 （ MT-12） from Naja naja atra venom on human bladder cancer cell EJ - m3 and study its mechanisms. Methods Using a specially constructed in vitro invasion chamber cells that penetrate the Matrigel were selected and cultured to establish offspring cell lines; The growth inhibition rates of EJ-m3 cell were investigated by MTY method with various concentration MT-12. chemokine receptor CXCR4 expressions were detected by flow cytometry after 24 hours with various concentration MT - 12. Results Acquire offspring cell lines nime EJ-m3 cell from bladder cancer cell EJ by selected again and a-gain. MT-12 effectively inhibited the growth of EJ-m3 cell, which depended on concentration of the drug. The IC50 after 72 hours with MT-12 on EJ-m3 cell were 0.66ug/mL, flow cytometry results indicated that the expression of CXCR4 gradually decreased with the increase of MT-12 dose（0.125-0.5ug/mL group, p =0. 020）. Conclusions MT-12 could significantly inhibit the growth of bladder cancer cells, The strong expression of CXCR4 might be one of its action mechanisms. MT-12 are largely potential anticaneer drug for bladder carcinoma.

关 键 词：膀胱肿瘤 肿瘤细胞 培养的 眼镜蛇毒液类 

分 类 号：R737.14[医药卫生—肿瘤]