VEGF、KDR和结核菌L型感染在前列腺癌中的表达及临床意义  

Expression and clinical significance of VEGF,KDR and infected with tubercular bacteria L-forms in prostate carcinoma

作　　者：赵芳芳[1] 汪万英[2] 俞岚[3] 

机构地区：[1]蚌埠医学院微生物教研室,安徽蚌埠233003 [2]蚌埠医学院第一附属医院中心实验室,安徽蚌埠233004 [3]蚌埠医学院病理教研室,安徽蚌埠233003

出　　处：《中国微生态学杂志》2009年第2期137-140,共4页Chinese Journal of Microecology

基　　金：安徽省教委自然科学基金资助(KJ2007B141)

摘　　要：目的探讨血管内皮生长因子(VEGF)及其受体VEGFR-2(KDR)和结核菌L型感染在前列腺肿瘤中的表达及临床相关性研究。方法应用免疫组化、原位杂交和抗酸染色等方法检测了65例前列腺癌(carcinomaof prostate,PCa)和30例良性前列腺增生(benign prostatic hyperplasia,BPH)中的VEGF、KDR蛋白及mRNA的表达,以及结核菌L型的检出率。并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果VEGF、KDR蛋白及mRNA阳性表达和结核菌L型检出率前列腺癌明显高于前列腺增生(P<0.001～0.05)。VEGF、KDR蛋白及mRNA阳性表达以及结核菌L型检出阳性率与前列腺癌的临床分期、病理分级差异有显著性(P<0.01～0.05)。结论VEGF及KDR蛋白及mRNA在前列腺肿瘤中有不同程度的异常表达,可能与结核菌L型感染有协同致瘤作用。提示2种基因均可作为判断前列腺癌生物学行为及患者预后参考指标。结核菌L型感染可能与前列腺癌的发生发展可能有一定关系,因此研究结核菌L型感染与前列腺癌的关系,具有重要的临床应用价值。Objective To study the expression of vascular endothelial growth factor, KDR acceptor and tubercular bacteria infection L-forms in prostate carcinoma and discuss the relationship between their expressions and clinical significance. Method The expression of VEGF,KDR protein and mRNA in 65 specimens from prostate carcinoma and 30 specimens from benign prostate hyperplasia were determined by immunohistochemical staining and Hybridization in situ. Gram＇s staining was used to examine the positive rate of tubercular bacteria L-forms in these tumors. The major clinical data and parameters of the pathological grades in prostate carcinoma were compared. χ^2 test was used for statistical analysis. Result Positive expressions of VEGF, KDR protein and mRNA in prostate carcinoma tissues were obviously higher than those of benign prostate hyperplasia. The examination rate of tubercular bacteria L-forms came to the same conclusion. The positive rate of expression of VEGF ,KDR protein,mRNA and the examination rate of infection by tubercular bacteria L-forms in prostate carcinoma had significantly correlation with their clinical classification and pathological grade（P 〈0.01-0.05）. Conclusion VEGF, KDR protein and mRNA have certain abnormal expressions in prostate tumors. Infection by tubercular bacteria L-forms perhaps has a concurrent oncogenic effect during the course of the occurrence and development of tumors. Both of the two genes could be useful markers for biological behavior of prostate tumors and prognosis of patients. To some extent, infection by tubercular bacteria L-forms has certain relationship with the occurrence and development of prostate carcinoma. Therefore ,study on the relationship between prostate carcinoma and tubercular bacteria L-forms can be valuable for clinical practice.

关 键 词：前列腺癌 基因 VEGF KDR 免疫组化 原位杂交 结核菌L型 

分 类 号：R737.25[医药卫生—肿瘤]