利用微卫星标记评估乌江彭水水电站对泉水鱼的遗传多样性影响  

Using Microsatellite Markers to Assess the Impacts by Pengshui Hydropower Station on Wujiang River on Population Genetic Diversity in Pseudogyrincheilus procheilus

作　　者：史方[1,2,3] 徐念[1,2,3] 熊美华[2] 王翔[2] 杨钟[2] 阙延福[2] 朱滨[2] 胡菊香[2] 常剑波[2] 

机构地区：[1]中国科学院水生生物研究所,湖北武汉430072 [2]水利部中国科学院水工程生态研究所,湖北武汉430079 [3]中国科学院研究生院,北京100049

出　　处：《水生态学杂志》2009年第2期117-121,共5页Journal of Hydroecology

基　　金：国家自然科学基金重大项目(30492034);乌江彭水水电站水生生物监测(PS/HB011-2006)

摘　　要：乌江彭水水电站建成后形成阻隔,可能对生境连通性及坝上坝下鱼类的交流产生不利,影响到物种遗传多样性和群体遗传结构。本研究通过磁珠富集法构建了泉水鱼(Pseudogyrincheilus procheilus)微卫星文库,建立了泉水鱼微卫星DNA分子标记体系,并利用这些微卫星分子标记初步评估了乌江彭水水电站对泉水鱼的遗传多样性影响。用6对具多态性的引物对乌江中35尾野生泉水鱼样本进行遗传多样性分析,结果显示,6个位点共检测到28个等位基因;每个位点平均杂合度为0.6536,平均多态信息含量为0.6085,平均Shannon多样性指数为1.2752;卡方检验得出2个位点显著偏离Hardy-Weinberg平衡,且其中1个位点中出现了无效等位基因。实验初步表明乌江流域泉水鱼的遗传多样性较为丰富,但可能受到了水电站阻隔的干扰。Fragmentation of the Wujiang river ecosystem caused by Pengshui hydropower station may disrupt the connectivity of ecosystem and communication of fish populations, consequently, fish population genetic structure and diversity will be affected. In this study, we established a partial genomic library for Pseudogyrincheilus procheilus. 6 microsatellite markers were assessed genetic diversity for 35 individuals. The results show that the total num- bers of alleles in 6 loci was twenty - eight. The average heterozygosity （ H ）, average polymorphism information content （PIC） and average Shannon＇s Information index （I） were 0. 6536, 0. 6085 and 1. 2752, respectively. Two loci showed significant deviation from Hardy-Weinberg equilibrium, and one of them had evidence for null alleles. The fndings indicated that although the genetic diversity of P. procheilus in Wujiang river was rich, it may had been infuenced by Pengshui hydropower station to a certain extent.

关 键 词：微卫星标记 水电站 泉水鱼 遗传多样性 

分 类 号：Q346.5[生物学—遗传学] X176[环境科学与工程—环境科学]