骨髓间充质干细胞移植对脑缺血区微血管增生及Notch1表达的影响  

Effects of bone marrow mesenchymal stem cells transplantation on angiogenesis and Notch1 expres-sion in cerebral ischemic area

作　　者：吕世刚[1] 祝新根[1] 张爱军[1] 涂伟[1] 娄远蕾[2] 阮琼芳[2] 汪泱[2] 邓志锋[1] 

机构地区：[1]南昌大学第二附属医院神经外科 [2]南昌大学第一附属医院泌尿外科研究所,330006

出　　处：《实验与检验医学》2009年第1期1-4,共4页Experimental and Laboratory Medicine

基　　金：国家自然科学基金资助项目(No30560156);江西省自然科学基金资助项目(No2007GZY1187)

摘　　要：目的观察骨髓间充质干细胞(BMSC)移植对脑缺血皮质区血管形成的影响及微血管Notch1表达的变化。方法用线栓法复制大鼠脑缺血再灌注损伤动物模型,随机分为假手术对照组、单纯脑缺血损伤组和脑缺血后BMSC移植组。采用免疫组织荧光染色法检测各组缺血皮质区Ⅷ因子及Notch1的表达。结果(1)植入的BMSC可以趋向性迁移到脑缺血损伤区域。(2)BMSC移植组脑缺血皮质区新生微血管密度较单纯脑缺血损伤组和假手术对照组明显增高。(3)BMSC移植组缺血皮质区多数微血管内皮细胞阳性表达Notch1,且明显多于单纯缺血组和假手术对照组。结论BMSC移植可促进脑缺血区血管新生,且可能与促进Notch1表达有关。Objective To observe the effects of bone marrow mesenchymal stem cells transplantation on angiogenesis and Notchl expression in cerebral isehemic area. MethOds Middle cerebral artery occlusion models were made by intraluminal thread method. Animals were divided into three groups randomly （cerebral ischemia group, BMSC transplantation group and sham operation control group）. Immunofluorescence methods were used to investigate the expressions of Factor Ⅷ and Notch1 in ischemic cortical areas. Results （1） The transplanted BMSC can move to cerebral ischemic regions. （2）The microvessel density in the injured cortical areas treated with BMSC were significantly higher than those of the cerebral ischemia group and sham operation control group. （3）The coexpression of Ⅷ and Notchl in injured cortical areas treated with BMSC were significantly higher than those of cerebral ischemia group and sham operation control group. Conclusion These results suggest that BMSC transplantation can improve the microvessel density in the injured cortical areas by promoting the expression of Notchl.

关 键 词：骨髓基质细胞 血管形成 脑缺血 NOTCH1 

分 类 号：R446.61[医药卫生—诊断学] R73-3[医药卫生—临床医学]