Stimulation of staphylococcal enterotoxin A combined with PML-RARα peptide on the specifical T-cells against NB4 cell line  

葡萄球菌肠毒素A联合PML-RARα多肽刺激特异性T细胞杀伤NB4细胞株的作用(英文)

作　　者：Chen Lin Xue Bai Lijian Yang Shaohua Chen B. N. Selvakumar Yangqiu Li 

机构地区：[1]Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou 510632, China [2]Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2009年第3期175-177,共3页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the Science and Technology Commission of Guangdong Province (No.06025169,No.2005B50301016);the Key Laboratory of Pathophysiology of Jinan University

摘　　要：Objective： To investigate the effects of staphyococcal enterotoxin A （SEA） on the cytotoxicity of T cells stimulated by PML-RARa peptide in vitro. Methods： Peripheral blood mononuclear cells （MNC） from healthy donor were obtained by density gradient centrifugation on Ficoll-Hypaque, MNC were cultured with PML-RARa peptide and SEA for 20 days. After induction, the cytotoxicity of T cells induced against NB4 and K562 cell lines were examined by Cell Counting Kit-8 （CCK-8）. The CD4 and CD8 surface markers on the harvested CD3^＋ T cells were detected by flow cytometry （FCM）. Results： The cytotoxicity of T cells induced by PML-RARa peptide with SEA was higher than that of T cells induced only by PML-RARa peptide against NB4 cells. The FCM assay showed that the ratio of CD4^＋/CD8^＋ T cells were gradually decreased in both groups of PML-RARα peptide whether with SEA or not at the intervals of day 5,10 and 20 day after induction, but the most significantly decreased by PML-RARe peptide with SEA. Conclusion： The specific cytotoxicity of T cells induced by PML-RARa peptide against NB4 cells could be enhanced with superantigen SEA.

关 键 词：SUPERANTIGEN PML-RARa pepUde NB4 cells T cells 

分 类 号：R392.11[医药卫生—免疫学] R378.11[医药卫生—基础医学]