葡萄糖激酶基因启动子区-30位点多态性与2型糖尿病的相关性研究  

Study of the correlation between the variation at -30 position of glucokinase gene promoter and type 2 diabetes

作　　者：马春宇[1] 王玉明[2] 

机构地区：[1]辽宁医学院实验诊断学教研室,辽宁锦州121001 [2]昆明医学院第一附属医院检验科,云南昆明650032

出　　处：《中国现代医学杂志》2009年第4期513-516,520,共5页China Journal of Modern Medicine

摘　　要：目的探讨葡萄糖激酶(glucokinase,GCK)基因启动子区-30位点多态性与2型糖尿病及其各项指标之间的关系。方法运用PCR-RFLP分析方法在389例无亲缘关系的个体中对GCK基因多态性进行检测。结果在2型糖尿病患者组中GG、GA和AA基因型频率分别为63.1%、31.9%和5.0%;健康对照者组中分别为66.4%、31.8%和1.8%。结论GCK基因启动子区-30位点多态性对2型糖尿病的发生不起主要作用,但两个变异等位基因同时存在时,发生2型糖尿病的年龄有所提前,而且可不伴有肥胖。[Objective] To investigate the association between the mutation at position -30 of the glucokinase gene promoter and type 2 diabetes and some relative index. [Methods] 389 unrelated subjects were studied by polymerase chain reaction-restriction fragment length polymorphism assay. [Result] Genotype frequency of GG, GA and AA was 63.1%, 31.9% and 5% in type 2 diabetes subjects and 66.4%, 31.8% and 1.8% in normal control subjects. [Conclusion] The variant at position -30 of the GCK gene promoter would not play a major role in the onset of type 2 diabetes, the onset year of type 2 diabetes would be earlier and the obesity would be not accompanied when the two alley gene exist in the same body.

关 键 词：葡萄糖激酶基因 2型糖尿病 启动子 变异 

分 类 号：R587.1[医药卫生—内分泌]