上皮性卵巢癌HIC1基因异常甲基化的检测及其临床意义的研究  

Promoter hypermethylation of HIC1 gene in epithelial ovarian cancer and its clinical significance

作　　者：沈文静[1] 戴冬秋[1] 郭科军[2] 王晓彩[3] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001 [2]中国医科大学附属第一医院妇科,辽宁沈阳110001 [3]沈阳市妇婴医院急诊科,辽宁沈阳110014

出　　处：《中国现代医学杂志》2009年第4期590-592,595,共4页China Journal of Modern Medicine

基　　金：辽宁省高等学校科研项目(No:2008839)

摘　　要：目的探讨HIC1基因异常甲基化在上皮性卵巢癌发生及发展中的作用。方法采用甲基化特异性PCR法检测63例上皮性卵巢癌、相应的盆腹腔转移灶41例及癌旁卵巢组织10例中HIC1基因甲基化状态。结果上皮性卵巢癌原发灶及转移灶中存在HIC1基因异常甲基化,发生率分别为33.3%及34.1%,显著高于正常卵巢组织(P<0.05)。HIC1基因甲基化在不同临床分期、分化程度及病理类型上皮性卵巢癌中无统计学差异(P>0.05)。结论HIC1基因异常甲基化与上皮性卵巢癌发生及发展相关。[Objective] To study the role of promoter hypermethylation of HIC1 gene in the course of tumorigenesis and progression of epithelial ovarian cancer. [Methods] Promoter hypermethylation of HIC1 gene was detected by methylation-specific PCP （MSP） in 63 epithelial ovarian cancer patients, corresponding 41 metastatic tissues of pelvic and abdomen cavity as well as 10 adjacent non-cancerous ovarian tissues. [Results] Promoter hypermethylation of HIC1 was detected in ovarian cancer tissues and metastatic sites, the frequency was 33.3% and 34.1% respectively, which was significantly higher than that in normal ovarian tissues （P 〈0.05）. No statistical difference of frequency of promoter hypermethylation of HIC1 was found between different tumor stages, different histological types and different differentiated grades （P 〉0.05）. [Conclusion] Promoter hypermethylation of HIC1 gene correlates with tumorigenesis and progression of epithelial ovarian cancer.

关 键 词：卵巢肿瘤 HIC1基因 肿瘤抑制基因 DNA甲基化 

分 类 号：R737.31[医药卫生—肿瘤]