VEGF-B及其受体Flt-1在肝癌中的表达及意义  

Expression and Significance of Vascular Endothelial Growth Factor B and Its Receptor Flt-1 in Hepatocellular Carcinoma

作　　者：唐瑞峰[1] 苑建磊[1] 齐帅[1] 田贵金[1] 孙超[1] 张志明[1] 李智峰[1] 

机构地区：[1]河北医科大学第四医院肝胆外科

出　　处：《中国肿瘤临床》2009年第5期267-271,共5页Chinese Journal of Clinical Oncology

基　　金：河北省卫生厅医学科学研究重点课题计划项目资助(编号:07116)

摘　　要：目的：探讨血管内皮生长因子B（vascular endothelial growth factorB，VEGF-B）及其受体Flt-1在肝癌中的表达及其与肝癌临床病理特征及预后的关系。方法：利用免疫组织化学染色法检测55例肝癌组织、33例癌旁肝组织及15例正常肝组织中VEG-B及Fit-1蛋白的表达情况，结合临床病理特征及预后对其进行统计学分析。结果：VEGF-B及Flt-1在肝癌组织中的表达率显著高于癌旁肝组织和正常肝组织中的表达率（P〈0．05）；有胆管或门静脉瘤栓形成的癌组织的VEGF-B及Flt-1的表达率显著高于无胆管或门静脉瘤栓形成组的表达率（P〈0．05）；VEGF-B及Flt-1的表达呈高度一致性（P〈0．05）；VEGF-B阳性组和Flt-1阳性组患者的1年、2年累积生存率均显著低于阴性组（P〈0．05）。结论：VEGF-B及其受体Flt-1与肝癌的生物学行为密切相关，并可作为肝癌患者预后的预测因素。Objective： To study the expression of vascular endothelial growth factor B （VEGF-B） and its receptor Fit-1 （vascular endothelial growth factor receptor 1, VEGFR-1） in primary liver cancer （PLC） and to determine if the presence of VEGF-B and Fit-1 is associated with the clinicopathological characteristics and prognosis of PLC. Methods： The expression of VEGF-B and Fit-1 proteins was examined by immunohisto- chemistry in 55 samples of PLC, 33 samples of tumor-adjacent tissue and 15 samples of normal liver tissue. Differences in distribution of VEGF-B and Fit-1 were analyzed using the X^2 test. Kaplan-Meier survival analysis was employed to estimate survival rate, and the Log-rank test was used to compare the difference in survival rates between the VEGF-B positive group and the VEGF-B negative group or between the Fit-1 positive group and the Fit-1 negative group. Results： Immunohistochemistry revealed that the positive rate of VEGF-B and Fit-1 staining in PLC tissues was higher than in tumor-adjacent tissues and normal liver tissues （P〈0.05）. Cancer tissues from patients with bile duct or portal vein tumor embolus presented with a higher positive rate of VEGF-B and Fit-1 staining than tissues from patients without bile duct or portal vein tumor embolus （P〈 0.01）. The 1- and 2-year survival rates in patients with positive expression of VEGF-B and Fit-1 were significantly lower than those in patients without expression of VEGF-B and Fit-1 （P〈0.05）. Conclusion： VEGF-B and Fit-1 are closely correlated with clinicopathological characteristics of PLC and may serve as prognostic factors for PLC.

关 键 词：原发性肝癌 血管内皮生长因子B 血管内皮生长因子受体1 累积生存率 

分 类 号：R735.7[医药卫生—肿瘤]