人乳头瘤病毒感染的宫颈上皮内瘤变中的hTERC基因分析  

Analysis on hTERC gene amplification in cervical intraepithelial neoplasia infected with human papillomavirus

作　　者：张艾芃[1] 李亚里[1] 张咏梅[2] 张全[1] 

机构地区：[1]中国人民解放军总医院妇产科,100853 [2]武警总医院妇产科

出　　处：《中国妇产科临床杂志》2009年第2期98-101,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：卫生部科研基金项目(WKJ2007-3-001)资助

摘　　要：目的探讨人乳头瘤病毒感染的宫颈上皮内瘤变细胞中3号染色体hTERC基因扩增情况。方法对57例病理诊断为CIN(CIN18例,CIN215例,CIN334例;其中包括HPV感染46例与无HPV感染11例)患者的液基细胞学检测剩余样本应用荧光原位杂交技术检测hTERC基因,同时以20例无HPV感染正常宫颈鳞状上皮作为对照,将结果与病理诊断进行比较。结果在正常宫颈鳞状上皮中,3号染色体hTERC基因主要表现为2个杂交荧光信号,宫颈病变与HPV感染的宫颈鳞状上皮中,出现了hTERC基因拷贝数增多,在CIN1～CIN3中,HPV感染率分别为12.5%、86.7%、94.1%,hTERC基因扩增的阳性率分别为25.0%、60.0%、82.3%,46例HPV感染的CIN中,hTERC基因扩增的阳性率为80.4%,而11例HPV阴性的CIN患者中,其扩增阳性率为18.2%,两组间比较,差异具有统计学意义(P<0.05)。结论3号染色体hTERC基因的扩增与HPV感染及宫颈病变程度相关,hTERC基因的扩增可能是HPV感染致端粒酶活性增加的早期事件。Objective To explore genomic amplification of the human telomerase gene （hTERC） in cervical intraepithelial neoplasia （CIN） with human papillomavirus （HPV） infection. Methods The hTERC gene was detected by fluorescence in situ hybridization （FISH） using 57 remaining samples after liquid - based cervical cytologi- cal test management, including 46 cases with HPV infection and 11 without. They were diagnosed with CIN1 in 8 cases, 15 CIN2, 34 CIN3. Twenty samples of normal cytology test without HPV infection served as the control group. Results In most of the normal squamous cervical cells, 2 signals of hTERC gene was detected, while gain of hTERC gene in the CIN1, CIN2 and CIN3 were 25.0%, 60.0% and 82. 3%, respectively, with HPV infection detected in 12.5%, 86.7% and 94. 1%, respectively. Amplification of hTERC gene in 46 cases with HPV infected was significant more than 11 cases without HPV infection, 80. 4% vs. 18. 2 %, （P〈0. 05）. Conclusions Amplification of hTERC gene on chromosome 3 may associate with severity of cervical disorders and HPV infection. The gain of hTERC gene may be an early stage event related to activation of telomerase resulted from HPV infection, the initial happening of the increased active of telomerase.

关 键 词：宫颈上皮内瘤变 人乳头瘤病毒 荧光原位杂交技术 HTERC基因 

分 类 号：R737.33[医药卫生—肿瘤] R752.53[医药卫生—临床医学]