Let-7a在人宫颈癌HeLa细胞不同周期时相的表达  

Time course of let-7a expression in the cell cycle of human cervical carcinoma HeLa cells

作　　者：周珏宇[1] 马文丽[1] 余海浪[1] 肖维威[1] 郑文岭[2] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515 [2]华南基因组研究中心,广东广州510800

出　　处：《南方医科大学学报》2009年第3期442-445,共4页Journal of Southern Medical University

基　　金：广东省生物芯片重点实验室基金(2004B60144)

摘　　要：目的探讨微小RNAlet-7a在HeLa细胞不同周期时相的差异表达。方法应用经典的双胸苷阻断法将HeLa细胞同步化于不同的周期时相,应用流式细胞术检测同步化的效率。应用实时荧光定量RT-PCR方法检测不同周期时相的HeLa细胞中let-7a的表达水平。结果HeLa细胞G1、S和G2/M期细胞同步化效率分别约为84.81%、83.65%和77.69%;let-7a在不同周期时相均有表达且存在差异,G1和S期表达水平较低,G2/M期表达水平较高。结论细胞周期对let-7a的表达有较大的影响,这为理解细胞周期调控的具体机制提供了新的线索。Objective To investigate the time course of let-7a microRNA expression in the cell cycle of HeLa cells. Methods HeLa cells were synchronized in G1, S and G2/M phases using double-thymidine block, and the cell cycle phases were defined by flow cytometry. Real-time quantitative RT-PCR was used to examine the expression oflet-7a in HeLa cells in different cell cycle phases. Results The synchronization rates of Gl, S and G2/M phases were 84.81%, 83.65% and 77.69%, respectively. Let-7a was constitutively expressed throughout the cell cycle in HeLa cells, but the expression levels in G~ and S phases were lower than those in G2/M phase. Conclusions Cell cycle can significantly influence the expression level of let-7a, which may provide new clues to the understanding of the cell cycle control mechanisms.

关 键 词：HELA细胞 微小RNA 细胞周期 同步化 

分 类 号：R737.33[医药卫生—肿瘤] Q2-33[医药卫生—临床医学]