实时荧光定量RT-PCR分析非小细胞肺癌SATB1的表达和临床病理意义  

Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer

作　　者：周来勇[1] 刘芳[2] 童健[1] 陈群请[1] 张福伟[1] 郭琳琅[3] 

机构地区：[1]南方医科大学珠江医院胸心外科,广东广州510282 [2]南方医科大学肿瘤研究所,广东广州510282 [3]南方医科大学珠江医院病理科,广东广州510282

出　　处：《南方医科大学学报》2009年第3期534-537,共4页Journal of Southern Medical University

摘　　要：目的检测非小细胞肺癌组织中SATB1 mRNA的表达,探讨SATB1表达与非小细胞肺癌发生、发展的关系及其临床病理意义。方法用TRIZOL提取非小细胞肺癌组织和正常肺组织总RNA后,将其反转录为cDNA,以实时荧光定量RT-PCR方法检测非小细胞肺癌组织及正常肺组织中SATB1 mRNA的表达,分析SATB1基因表达与临床病理参数的相关性。结果癌组织中SATB1 mRNA表达量为正常组织13倍,两者差异显著(P<0.001),其中有、无转移组分别为正常组织23.63倍和5.57倍。结论SATB1 mRNA的表达水平可能与非小细胞肺癌发生发展及淋巴转移有关,有望成为判断非小细胞肺癌预后的一个指标。Objective To detect the expression of AT-rich sequence-binding protein （SATB1） mRNA in non-small cell lung cancer （NSCLC） and explore the role of SATB1 in the development of NSCLC. Methods The total RNA was extracted from NSCLC tissues and normal lung tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of SATB 1 mRNA these tissues. Results The expression of SATB 1 mRNA was 13-fold higher in NSCLC tissues than in normal lung tissues （P〈0.001）, and in metastatic and nonmetastatic NSCLC, the expression was 23.63 and 5.57 folds that in normal lung tissues, respectively. Conclusion SATB1 mRNA expression might be associated with the development and lymph node metastasis of NSCLC and may potentially used as an indicator for predicting the prognosis of NSCLC.

关 键 词：非小细胞肺癌 SATB1 mRNA 实时荧光定量RT-PCR 

分 类 号：R734.2[医药卫生—肿瘤]