肠胃清药物血清对人结肠癌细胞株HCT-8/V多药耐药的逆转作用及机制研究  

Reversal of Multidrug Resistance of Human Intestinal Cancer Cell Line HCT-8/V and its Mechanism by Changweiqing Serum

作　　者：鲍文磊[1] 孙珏[1] 范忠泽[1] 许建华[1] 邓皖利[1] 

机构地区：[1]上海中医药大学附属普陀医院肿瘤科,上海200062

出　　处：《辽宁中医杂志》2009年第3期464-466,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30672683);上海市教委资助项目(05CZ16)

摘　　要：目的:研究肠胃清方对大肠癌耐药细胞株HCT-8/V的逆转作用及其抑制P-gp表达的影响。方法:MTT法测定肠胃清对HCT-8/V细胞株的逆转作用,流式细胞术测定P糖蛋白(P-gp)的表达。结果:HCT-8/V细胞株对VCR、DDP、5-FU、MMC的耐药指数分别为18.6、22.3、4.3、3.7。经肠胃清方药物血清作用48h后,其对化疗的耐药性显著下降,对VCR、DDP的逆转指数分别为3.3和7.5,静息状态下HCT-8/V细胞的P-gp表达显著高于HCT-8(P<0.01),而经肠胃清药物血清作用后P-gp表达显著降低(P<0.01)。结论:肠胃清方可以逆转人大肠癌耐药细胞株HCT-8/V的多药耐药性,其机制可能与其下调P-gp的表达有关。Objective ：To investigate the reversal effect of Changweiqing on multidrug resistance of human intestinal cancer cell line HCT - 8/V and the impact of Changweiqing to the expressions of P - gp in HCT - 8/V. Methods ：The reversal effect of Changweiqing was assayed by MTT assay, and the expressions of P - gp in HCT - 8/V cell line was detected with flow cytometry （ FCM ）. Results ： The resistance factor（RF） of HCT - 8/V cells to VCR, DDP, 5 - FU, MMC respectively were 18. 6,22. 3,4. 3, 3.7. After simultaneous treatment 48 hours with Changweiqing serum, the sensitivity of HCT - 8/V rose signifieandy to VCR, DDP. The reversal index （RI） to VCR, DDP was 3. 3,7. 5. The P - gp had higher expression in HCT -8/V than in HCT -8 （P 〈 0.01 ）. It showed a low level after Changweiqing treatment. Conclusion ： Changweiqing can reverse the resistance of HCT - 8/V cell line. The mechanism may be related to the effect of down - regulation P - gp.

关 键 词：肠胃清方 多药耐药 大肠癌 P糖蛋白 

分 类 号：R735.35[医药卫生—肿瘤]