紫杉醇联合三氧化二砷作用人结肠腺癌LS-174T细胞增殖及凋亡的实验研究  

The study of the proliferation and apoptosis of human colorectal cancer cell line LS-174T treated by paclitate and arsenic trioxide

作　　者：刘洪义[1] 王权[1] 于金海[1] 王大广[1] 所剑[1] 

机构地区：[1]吉林大学第一医院普外科,吉林长春130021

出　　处：《中国实验诊断学》2009年第3期324-327,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的以人结肠腺癌LS-174T细胞系作为体外模型,研究紫杉醇与三氧化二砷联合作用抑制肿瘤细胞增殖及凋亡双重作用的机理。方法采用不同浓度的紫杉醇与三氧化二砷联合作用于体外培养的人结肠腺癌LS-174T细胞,以MTT比色法和形态学观察检测其对人结肠腺癌LS-174T细胞的抑制作用。以TUNEL法和AnnexinⅤ-FITC、PI染色、共聚焦显微镜检测其对人结肠腺癌LS-174T细胞系的凋亡诱导作用。结果不同浓度的紫杉醇与三氧化二砷联合组对人结肠腺癌LS-174T细胞增殖的抑制作用均强于紫杉醇或三氧化二砷单用药组。联合用药组从形态学观察及应用TUNEL法和AnnexinⅤ-FITC、PI染色结果显示联合用药组可检测到大量凋亡结肠腺癌细胞,明显多于单用药组。结论紫杉醇与三氧化二砷联合应用于人结肠腺癌LS-174T细胞可协同抑制其增殖,并可明显诱导肿瘤细胞凋亡发生。Objective To investigate the function of Paclitaxe （Taxol） combining with arsenic trioxide （As2O3） on human colorectal cancer cell line LS-174T.Methods Human colorectal cancer cell line LS-174T was treated with Paclitaxe （Taxol） at different concentrations （1,5,10 μmol/L） in vitro,combining with 1,2,5 μmol/L arsenic trioxide （As2O3）. The function of inhibition was observed by MTr reduction assay （Mononuclear cell direc： cytotoxicity assay）,immunohistochemistry and light microscope. The inducing apoptosis action of the cell line LS- 174T was tested by TUNEL.Annexin V-FTTC.PI staining.confceal microscopy. Results 1,5,10 μmol/L Taxol combining with 1,2,5 μmol/L As2O3 had a stronger inhibition function on human colorectal cancer cell line LS-174T than 1,5,10 μmol/L Taxol or 1,2,5 μmol/L As2O3. After 5 μmol/L Taxol and 2 μmol/L As2O3 were applied 48 hours,we could observe stronger inhibition function than 5 μmol/L Taxol or 2 μmol/L As2O3 individually applied from morphology （P 〈 0.05）, and many apepotosis cell could be seen throught TUNEL. Annexin V-FITC. PI staining, confocal microscopy. Conclusion Paclitaxe （Taxol） combining with arsenic trioxide （As2O3） can definitely inhibit the growth of eolorectal cancer cell line I.S-174T in vitro,and the inducing apoptosis action was observed too.

关 键 词：紫杉醇 三氧化二砷 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]