乙酰丙酮-甲醛为荧光衍生试剂同步荧光法测定胺类物质  

Synchronous fluorimetric determination of primary amine-containing compounds using acetylacetone-formaldehyde as the fluorigenic reagent

作　　者：马丽英[1] 姜吉刚[1] 冯霞光[2] 王怀友[2] 

机构地区：[1]滨州医学院化学教研室,山东烟台264003 [2]山东师范大学化学化工与材料科学学院,山东济南250014

出　　处：《化学工程师》2009年第3期24-26,共3页Chemical Engineer

基　　金：山东省自然科学基金项目(Y2006B31)

摘　　要：在用乙酰丙酮-甲醛作荧光衍生试剂测定胺类物质时,由于试剂背景干扰,胺类物质无法准确测定。本文以盐酸普鲁卡因为例,研究了同步荧光光度法测定胺类物质消除背景干扰的条件。同法测定了苯丙氨酸、正丙氨、正丁氨、谷氨酸、色氨酸和亮氨酸,给出了上述物质的线性回归方程,检出限,回收率和分析条件。Because of the interference of background of acetylacetone-formaldehyde, some primary aminecontaining compounds cannot be determined by normal fluorimetry using acetylacetone-formaldehyde as the fluorigenic reagent. The procaine hydrochloride was studied as a sample in the synchronous fluorimetry using acetylacetone-formaldehyde as the fluofigenic reagent and the background of acetylacetone-formaldehyde was eliminated without separation. Some primary amine-containing compounds, such as procaine hydrochloride, L-phenylalanine, n-propylamine, n-butylamine, L-Glutamic acid, DL-trytophan and L-leucine can be determined by synchronous fluorimetry. The linear regression equations of calibration graph, detection limits, recoveries and optimum condition were listed.

关 键 词：乙酰丙酮-甲醛 同步荧光 盐酸普鲁卡因 伯胺 

分 类 号：O657.34[理学—分析化学]