贮藏温度和时间对麻疯树花粉生活力的影响  

Effects of Different Preservation Temperature and Time on Jatropha curcas Pollen Viability

作　　者：雷宝盛[1] 周兰英[1] 董会[1] 袁定昌[2] 向倩[1] 

机构地区：[1]四川农业大学林学园艺学院,四川雅安625014 [2]北京林业大学

出　　处：《林业科技开发》2009年第2期20-22,共3页China Forestry Science and Technology

基　　金：国家科技支撑课题“麻疯树高油、高产、多抗性良种选育技术研究”(编号:2007BAD5OB01)

摘　　要：为保证麻疯树杂交的正常开展,花粉贮藏和生活力测定是必需的。采用联苯胺染色法分析了麻疯树花粉在3种贮藏温度下,不同时间段的活力变化。结果表明:麻疯树盛开的花粉生活力可达93.4%;整体上花粉生活力随贮藏时间的增加呈逐渐下降的趋势;贮藏方法以冷冻(-24～-18℃)最佳,冷藏(0～4℃)次之,常温条件下花粉生活力下降最快,3种保存条件下花粉生活力存在明显差异。To determine the vitality of pollen in different preservation condition is so necessary, that crossing of Jatropha curcas could be carrying out normally. The changes of Jatropha curcas pollen viability in three storage temperatures and different storage periods were analyzed by a method of benzidine staining in this experiment. The results showed that the pollen vitality of Jatropha curcas achieved 93.4% in full bloom and then gradually declined. The pollen stored at -24 - - 18℃ kept higher vitality than the chilled pollen （0 - 4℃ ）, and the pollen vitality kept at room temperature decreased at the fastest speed. The effects of different storage methods on pollen vitality were significant.

关 键 词：麻疯树 花粉 生活力 贮藏方法 

分 类 号：S667.9[农业科学—果树学] S816.8[农业科学—园艺学]