基因工程菌漆酶对蒽醌染料的脱色研究  

Decolorization of Anthraquinone Dye by Laccase-secreting Engineered Strain

作　　者：郭梅[1] 路福平[2] 刘敏尧 李拖平[1] 梁鹏[1] 蒲军[2] 

机构地区：[1]天津农学院食品科学系,天津300384 [2]天津科技大学生物工程学院工业微生物重点实验室,天津300222 [3]天津诺奥科技发展有限公司,天津300384

出　　处：《环境科学与技术》2009年第4期133-136,共4页Environmental Science & Technology

基　　金：天津市自然科学基金重点项目(07JCZDJC03100)

摘　　要：利用基因工程菌甲醇毕赤酵母(PMAD16/pMETαA-Lcc1)培养产生的重组漆酶,研究了重组漆酶对蒽醌染料Remazol Brilliant Blue R脱色的影响。结果表明:重组漆酶对蒽醌染料RBBR脱色的最佳pH值为4.5,温度为45℃,当溶液中漆酶活力为10.0U/mL时,在最适脱色条件下作用14h,粗漆酶和纯化漆酶对蒽醌染料RBBR(80mg/L)的脱色率分别为95.2%和87.5%,重组漆酶可有效地使蒽醌染料RBBR脱色。Impacts of recombinant laccase from gene engineered strain Pichia methanolica on the anthraquinone dye Remazol Brilliant Blue R （RBBR） were studied. Results showed that under the optimal decolorizing pH and temperature of 4.5 and 45℃ respectively, crude and purified laccase decolorization for 80mg ·L-1 RBBR after incubation for 14h with 10.0U ·mL-1 of laccase added were 95.2% and 87.5% respectively. The recombinant laccase could efficiently decolorize anthraquinone dye RBBR.

关 键 词：基因工程菌 重组漆酶 蒽醌染料RBBR 脱色 

分 类 号：X79[环境科学与工程—环境工程]