活动性系统性红斑狼疮自噬及相关基因研究  

The expression of autophagy and related genes in patients with active systemic lupus erythematosus

作　　者：赵继军[1] 李美荣[2] 陆才生[1] 古洁若[1] 潘云峰[1] 余步云[1] 

机构地区：[1]中山大学附属第三医院风湿免疫科,广州510630 [2]中山大学附属第三医院皮肤科,广州510630

出　　处：《中华风湿病学杂志》2009年第3期148-151,共4页Chinese Journal of Rheumatology

基　　金：基金项目：国家自然科学基金（30571736/C0302050301）;广东省科技计划资助项目（2006835601003）

摘　　要：目的观察活动性系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）的自噬现象并检测相关基因的表达水平。方法以初诊及新发作的活动性SLE患者20例为病例组，类风湿关节炎（RA）患者10例和健康志愿者10名分别作为对照组。密度梯度离心法分离PBMC，并利用贴壁法除去单核细胞。运用透射电镜（TEM）观察细胞的超微结构，半定量反转录一聚合酶链反应（RT—PCR）及实时定量RT—PCR检测各组PBMC中Beclinl、微管相关蛋白1轻链3（MAPLC3）mRNA的表达水平。结果TEM发现活动性SLE患者PBMC中存在自噬现象；Beclinl和MAPLC3mRNA在活动性SLE患者表达量均增高，且分别较RA组和健康对照组表达差异有统计学意义。结论活动性SLE患者外周血PBMC存在自噬现象，且细胞白噬相关基因表达较健康对照组和RA组增强，这可能与SLE的发生、发展相关。Objective To investigate the autophagy and the expression of its related genes in the peripheral blood mononuclear cells （ PBMCs ） of active systemic lupus erythematosus （ SLE ） patients. Methods Patients with newly onset or recently-diagnosed SLE （n=20） were enrolled. RA patients （n=10） and healthy blood donors （n=10） were used as controls. PBMCs from all subjects were immediately isolated by Ficoll- Hypaque density gradient centrifugation. And then monocytes were removed by wall sticking method. The morphology was observed using transmission electron microscopy （TEM）. Messenger RNA （mRNA） expression of Beclin 1 and microtubule-associated protein l-light chain 3 （MAPLC3） were determined by reverse transcription polymerase chain reaction （RT-PCR） and real-time quantitative RT-PCR respectively. Results TEM showed autophagic phenomenon in PBMCs from active SLE. On the mRNA level, expression of Beclin 1 and LC3 was significantly increased in fresh isolated SLE cells as compared with RA or healthy donor＇s PBMCs. Conclusion Based on these results, we can conclude that autophagy occurs in active SLE and the expression of its related genes is significantly higher in active SLE than in RA or normal controls. The enhanced autophagy may indicate its role in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 外周血单个核细胞 微管相关蛋白质类 BECLINL 

分 类 号：R686[医药卫生—骨科学]